Small Angle Scattering Biological Data Bank SASBDB

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SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	37	nm³

SASDHY5 – In cellulo luciferase protein crystals recombinantly expressed within High Five insect cells

Photinus pyralis firefly luciferase experimental SAS data

Photinus pyralis firefly luciferase Kratky plot

Sample:	Photinus pyralis firefly luciferase, 61 kDa Photinus pyralis protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH26 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH36 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV)

mock recombinant baculovirus experimental SAS data

mock recombinant baculovirus Kratky plot

Sample:	Mock recombinant baculovirus, 0 kDa unidentified baculovirus
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH56 – High Five insect cell culture (control; uninfected)

High Five insect cells experimental SAS data

Sample:	High Five insect cells, 0 kDa Trichoplusia ni
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH66 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH76 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells (cell-culture serial dilution series)

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Dec 3

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

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