Small Angle Scattering Biological Data Bank SASBDB

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SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

Myotilin (222-452) experimental SAS data

Sample:	Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	36	nm³

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

Myotilin (222-452) R405K experimental SAS data

Sample:	Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	36	nm³

SASDJT2 – DNA-directed RNA polymerase (RPO41)

DNA-directed RNA polymerase experimental SAS data

Sample:	DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein
Buffer:	40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Sep 25

PfKsgA1 functions as a transcription initiation factor and interacts with the N-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. Int J Parasitol (2020)
Gupta A, Shrivastava D, Shakya AK, Gupta K, Pratap JV, Habib S

R_g^Guinier	4.4	nm
D_max	12.5	nm
Volume^Porod	295	nm³

SASDJU2 – DNA-directed RNA polymerase (RPO41) in complex with rRNA adenine N(6)-methyltransferase (PfKsgA1)

DNA-directed RNA polymeraserRNA adenine N (6) methyl transferase experimental SAS data

DNA-directed RNA polymerase rRNA adenine N (6) methyl transferase Kratky plot

Sample:	DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein RRNA adenine N (6) methyl transferase monomer, 39 kDa Plasmodium falciparum protein
Buffer:	40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2020 Jan 10

R_g^Guinier	5.5	nm
D_max	15.0	nm
Volume^Porod	393	nm³

SASDJV2 – DNA-directed RNA polymerase (RPO41) in complex with mitochondrial transcription factor 1 (MTF1)

DNA-directed RNA polymeraseMitochondrial transcription factor 1 experimental SAS data

DNA-directed RNA polymerase Mitochondrial transcription factor 1 Kratky plot

Sample:	DNA-directed RNA polymerase monomer, 150 kDa Saccharomyces cerevisiae protein Mitochondrial transcription factor 1 monomer, 40 kDa Saccharomyces cerevisiae protein
Buffer:	40 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM DDT, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2019 Dec 7

R_g^Guinier	4.5	nm
D_max	14.4	nm
Volume^Porod	292	nm³

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