Browse by MODEL: No model

SASDFH9 – Poly-adenosine rA30 in 5 mM MgCl2

Poly-adenosine experimental SAS data
Poly-adenosine Kratky plot
Sample: Poly-adenosine monomer, 10 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFM9 – Poly-uridine rU30 in 400 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 400 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.5 nm
VolumePorod 14 nm3

SASDFN9 – Poly-uridine rU30 in 600 mM NaCl

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 600 mM NaCl, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 10.0 nm
VolumePorod 14 nm3

SASDFR9 – Poly-uridine rU30 in 5 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 5 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 15 nm3

SASDFS9 – Poly-uridine rU30 in 10 mM MgCl2

Poly-uridine experimental SAS data
Poly-uridine Kratky plot
Sample: Poly-uridine monomer, 9 kDa RNA
Buffer: 1 mM Na-MOPS, 20 mM NaCl, 10 mM MgCl2, 20 µM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 24
Visualizing disordered single-stranded RNA: connecting sequence, structure and electrostatics. J Am Chem Soc (2019)
Plumridge A, Andresen K, Pollack L
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 14 nm3

SASDE62 – Ribonuclease E from Yersinia pestis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 248 kDa Yersinia pestis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 16.4 nm
VolumePorod 470 nm3

SASDE72 – Ribonuclease E from Francisella tularensis

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 256 kDa Francisella tularensis protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.1 nm
Dmax 17.2 nm
VolumePorod 491 nm3

SASDE82 – Ribonuclease E from Burkholderia pseudomallei

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 250 kDa Burkholderia pseudomallei protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 4.8 nm
Dmax 14.9 nm
VolumePorod 437 nm3

SASDE92 – Ribonuclease E from Acinetobacter baumannii

Endoribonuclease E experimental SAS data
Endoribonuclease E Kratky plot
Sample: Endoribonuclease E tetramer, 254 kDa Acinetobacter baumannii protein
Buffer: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Feb 11
A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties. Sci Rep 9(1):7952 (2019)
Mardle CE, Shakespeare TJ, Butt LE, Goddard LR, Gowers DM, Atkins HS, Vincent HA, Callaghan AJ
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 508 nm3

SASDFK8 – GON7, the fifth subunit of human KEOPS

EKC/KEOPS complex subunit GON7 experimental SAS data
EKC/KEOPS complex subunit GON7 Kratky plot
Sample: EKC/KEOPS complex subunit GON7 monomer, 13 kDa Homo sapiens protein
Buffer: 20 mM MES, 200 mM NaCl, 5 mM β-mercaptoethanol, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Mar 26
Defects in t6A tRNA modification due to GON7 and YRDC mutations lead to Galloway-Mowat syndrome. Nat Commun 10(1):3967 (2019)
Arrondel C, Missoury S, Snoek R, Patat J, Menara G, Collinet B, Liger D, Durand D, Gribouval O, Boyer O, Buscara L, Martin G, Machuca E, Nevo F, Lescop E, Braun DA, Boschat AC, Sanquer S, Guerrera IC, Revy P, Parisot M, Masson C, Boddaert N, Charbit M, Decramer S, Novo R, Macher MA, Ranchin B, Bacchetta J, Laurent A, Collardeau-Frachon S, van Eerde AM, Hildebrandt F, Magen D, Antignac C, van Tilbeurgh H, Mollet G
RgGuinier 3.1 nm
Dmax 12.5 nm
VolumePorod 46 nm3