Browse by MODEL: No model

SASDBQ5 – Cyclohexanone monooxygenase, NADP+, N497A

Cyclohexanone monooxygenase experimental SAS data
Cyclohexanone monooxygenase Kratky plot
Sample: Cyclohexanone monooxygenase monomer, 61 kDa Rhodococcus sp. HI-31 protein
Buffer: 50 mM Tris 5 mM NADP+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Jul 15
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.7 nm
Dmax 9.2 nm
VolumePorod 100 nm3

SASDBR5 – Cyclohexanone monooxygenase, K501A

Cyclohexanone monooxygenase experimental SAS data
Cyclohexanone monooxygenase Kratky plot
Sample: Cyclohexanone monooxygenase monomer, 61 kDa Rhodococcus sp. HI-31 protein
Buffer: 50 mM Tris, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Jul 15
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 3.0 nm
Dmax 10.1 nm
VolumePorod 120 nm3

SASDBS5 – Cyclohexanone monooxygenase, N497A-K501A

Cyclohexanone monooxygenase experimental SAS data
Cyclohexanone monooxygenase Kratky plot
Sample: Cyclohexanone monooxygenase monomer, 61 kDa Rhodococcus sp. HI-31 protein
Buffer: 50 mM Tris 5 mM NADP+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Jul 15
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 3.0 nm
Dmax 11.0 nm
VolumePorod 130 nm3

SASDBT5 – Cyclohexanone monooxygenase, NADP+, N497A-K501A

Cyclohexanone monooxygenase experimental SAS data
Cyclohexanone monooxygenase Kratky plot
Sample: Cyclohexanone monooxygenase monomer, 61 kDa Rhodococcus sp. HI-31 protein
Buffer: 50 mM Tris 5 mM NADP+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Jul 15
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 110 nm3

SASDBU5 – Cyclopentadecanone monooxygenase, wild-type

Cyclopentadecanone 1,2-monooxygenase experimental SAS data
Cyclopentadecanone 1,2-monooxygenase Kratky plot
Sample: Cyclopentadecanone 1,2-monooxygenase monomer, 68 kDa Pseudomonas sp. HI-70 protein
Buffer: 50 mM Tris 2 mM TCEP, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2013 Jan 27
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.8 nm
Dmax 10.4 nm
VolumePorod 110 nm3

SASDBV5 – Cyclopentadecanone monooxygenase, NADP+, wild-type

Cyclopentadecanone 1,2-monooxygenase experimental SAS data
Cyclopentadecanone 1,2-monooxygenase Kratky plot
Sample: Cyclopentadecanone 1,2-monooxygenase monomer, 68 kDa Pseudomonas sp. HI-70 protein
Buffer: 50 mM Tris 2 mM TCEP 5 mM NADP+, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 2
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 120 nm3

SASDBW5 – Cyclopentadecanone monooxygenase, NADP+ and cyclopentadecanone, wild-type

Cyclopentadecanone 1,2-monooxygenase experimental SAS data
Cyclopentadecanone 1,2-monooxygenase Kratky plot
Sample: Cyclopentadecanone 1,2-monooxygenase monomer, 68 kDa Pseudomonas sp. HI-70 protein
Buffer: 50mM Tris 2mM TCEP 5mM NADP+ 1mM cyclopentadecanon, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 2
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 110 nm3

SASDBX5 – Cyclopentadecanone monooxygenase, NADP+ and ω-pentadecalactone, wild-type

Cyclopentadecanone 1,2-monooxygenase experimental SAS data
Cyclopentadecanone 1,2-monooxygenase Kratky plot
Sample: Cyclopentadecanone 1,2-monooxygenase monomer, 68 kDa Pseudomonas sp. HI-70 protein
Buffer: 50mM Tris mM TCEP 5mM NADP+ 1mM ω-pentadecalactone, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 2
The role of conformational flexibility in Baeyer-Villiger monooxygenase catalysis and structure. Biochim Biophys Acta 1864(12):1641-1648 (2016)
Yachnin BJ, Lau PCK, Berghuis AM
RgGuinier 2.6 nm
Dmax 9.0 nm
VolumePorod 100 nm3

SASDB27 – Chimeric EcRHH-RcPutA: The E.coli Proline utilization A RHH domain fused to R.capsulatus PutA

Proline utilization A experimental SAS data
Proline utilization A Kratky plot
Sample: Proline utilization A dimer, 251 kDa Escherchia coli, Rhodobacter … protein
Buffer: 50 mM Tris, 200 mM NaCl, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2012 Oct 16
Engineering a trifunctional proline utilization A chimaera by fusing a DNA-binding domain to a bifunctional PutA. Biosci Rep 36(6) (2016)
Arentson BW, Hayes EL, Zhu W, Singh H, Tanner JJ, Becker DF
RgGuinier 5.2 nm
Dmax 18.3 nm
VolumePorod 308 nm3

SASDAW7 – Aptamer AIR-3A 2'FU

AIR-3A 2'FU experimental SAS data
AIR-3A 2'FU Kratky plot
Sample: AIR-3A 2'FU dimer, 13 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 12 nm3