Structure and target interaction of a G-quadruplex RNA-aptamer.

Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RNA Biol 13(10):973-987 (2016 Oct 2)

PMID: 27471797 doi: 10.1080/15476286.2016.1212151

Published in SASBDB: 2016 Sep 8

SASDAT7 – Aptamer AIR-3 5FU Sample: AIR3-5FU dimer, 69 kDa RNA Buffer: water, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 4.3 nm Dmax 16.0 nm VolumePorod 77 nm3

SASDAU7 – Aptamer AIR-3 FdU Sample: AIR3-FdU dimer, 69 kDa RNA Buffer: water, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 5.0 nm Dmax 16.0 nm VolumePorod 67 nm3

SASDAV7 – Aptamer AIR-3A Sample: AIR-3A dimer, 13 kDa RNA Buffer: water, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 1.9 nm Dmax 6.5 nm VolumePorod 14 nm3

SASDAW7 – Aptamer AIR-3A 2'FU Sample: AIR-3A 2'FU dimer, 13 kDa RNA Buffer: water, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 1.9 nm Dmax 6.0 nm VolumePorod 12 nm3

SASDAX7 – Aptamer AIR-3A G18U Sample: AIR-3A G18U tetramer, 25 kDa RNA Buffer: water, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6 RgGuinier 2.5 nm Dmax 8.3 nm VolumePorod 24 nm3

SASDAY7 – IL-6R tetramer-dimer equilibrium Sample: Interleukin-6 receptor subunit alpha tetramer, 164 kDa Homo sapiens protein Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein Buffer: water, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Oct 1 RgGuinier 4.9 nm Dmax 20.0 nm VolumePorod 240 nm3

SASDAZ7 – IL-6R AIR-3A 2:4 complex Sample: AIR-3A tetramer, 26 kDa RNA Interleukin-6 receptor subunit alpha dimer, 82 kDa Homo sapiens protein Buffer: water, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2014 Oct 1 RgGuinier 6.7 nm Dmax 25.0 nm VolumePorod 150 nm3