Browse by MODEL: No model

SASDQA8 – Rap guanine nucleotide exchange factor Epac1

Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 130 nm3

SASDQB8 – Rap guanine nucleotide exchange factor Epac1 with cAMP

Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.5 nm
Dmax 13.0 nm
VolumePorod 140 nm3

SASDQC8 – Rap guanine nucleotide exchange factor Epac1 with CE3F4 inhibitor

Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.3 nm
Dmax 11.4 nm
VolumePorod 140 nm3

SASDQD8 – Rap guanine nucleotide exchange factor Epac1 with cAMP and CE3F4 inhibitor

Rap guanine nucleotide exchange factor 3 experimental SAS data
Rap guanine nucleotide exchange factor 3 Kratky plot
Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4
Membranes prime the RapGEF EPAC1 to transduce cAMP signaling Nature Communications 14(1) (2023)
Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
RgGuinier 3.3 nm
Dmax 11.8 nm
VolumePorod 134 nm3

SASDRF6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated EphB4 peptide

Ephrin type-B receptor 4Ras GTPase-activating protein 1 experimental SAS data
Ephrin type-B receptor 4 Ras GTPase-activating protein 1 Kratky plot
Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein
Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 176 nm3

SASDRG6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated p190RhoGAP peptide

Rho GTPase-activating protein 35Ras GTPase-activating protein 1 experimental SAS data
Rho GTPase-activating protein 35 Ras GTPase-activating protein 1 Kratky plot
Sample: Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 15.1 nm
VolumePorod 194 nm3

SASDRH6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to a doubly phosphorylated Dok1 peptide

Ras GTPase-activating protein 1Docking protein 1 experimental SAS data
Ras GTPase-activating protein 1 Docking protein 1 Kratky plot
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Docking protein 1 monomer, 3 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2021 Nov 4
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 14.6 nm
VolumePorod 196 nm3

SASDRJ6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP)

Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.5 nm
Dmax 9.8 nm
VolumePorod 42 nm3

SASDRK6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated EphB4 peptide

Ephrin type-B receptor 4Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Ephrin type-B receptor 4 Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.7 nm
Dmax 10.5 nm
VolumePorod 44 nm3

SASDRL6 – SH2-SH3-SH2 domains of Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated p190RhoGAP peptide

Rho GTPase-activating protein 35Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Rho GTPase-activating protein 35 Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Rho GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8, 350 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Dec 12
Diverse p120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.4 nm
Dmax 7.7 nm
VolumePorod 44 nm3