Small Angle Scattering Biological Data Bank SASBDB

MACROMOLECULE TYPE: heterocomplex

SASDGB3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 4.5)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E34K experimental SAS data

80bp_DNA Forward 80bp_DNA Reverse DNA-binding protein HU-alpha, E34K Kratky plot

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer:	10 mM sodium acetate, 50 mM NaCl, pH: 4.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling. Nat Commun 11(1):2905 (2020)
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M

SASDGC3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 5.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K dimer, 19 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 50 mM NaCl, pH: 5.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

SASDGD3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 6.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K, 100 kDa Escherichia coli protein
Buffer:	10mM Bis-Tris, 50 mM NaCl, pH: 6.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

R_g^Guinier	7.3	nm
D_max	28.0	nm
Volume^Porod	330	nm³

SASDGE3 – DNA-binding protein HU-alpha, E34K mutant bound to 80 bp DNA (pH 7.5)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E34K, 100 kDa Escherichia coli protein
Buffer:	10 mM Bis-Tris, 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Nov 2

R_g^Guinier	7.1	nm
D_max	27.5	nm
Volume^Porod	309	nm³

SASDG85 – Wild-type full-length Liver Receptor Homolog-1/Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha peptide complex bound to the CYP7A1 promoter oligonucleotide duplex.

Liver Receptor Homolog-1Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 AlphaCYP7A1 Promoter ForwardCYP7A1 Promoter Reverse experimental SAS data

Sample:	Liver Receptor Homolog-1 monomer, 64 kDa Homo sapiens protein Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha monomer, 2 kDa Homo sapiens protein CYP7A1 Promoter Forward monomer, 4 kDa Homo sapiens DNA CYP7A1 Promoter Reverse monomer, 4 kDa Homo sapiens DNA
Buffer:	20 mM TRIS, 150 mM NaCl, 2% v/v glycerol, 0.5 mM CHAPS, 5 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 26

Integrated Structural Modeling of Full-Length LRH-1 Reveals Inter-domain Interactions Contribute to Receptor Structure and Function. Structure (2020)
Seacrist CD, Kuenze G, Hoffmann RM, Moeller BE, Burke JE, Meiler J, Blind RD

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	76	nm³

SASDHT9 – Dimeric NanR-DNA hetero-complex from Escherichia coli

HTH-type transcriptional repressor NanR(GGTATA)2 repeat DNA experimental SAS data

Sample:	HTH-type transcriptional repressor NanR dimer, 59 kDa Escherichia coli protein (GGTATA)2 repeat DNA monomer, 11 kDa DNA
Buffer:	20 mM Tris, 150 mM NaCl, 0.1 % (w/v) sodium azide, pH: 8
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Apr 20

Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism (2020)
Horne C, Venugopal H, Panjikar S, Henrickson A, Brookes E, North R, Murphy J, Friemann R, Griffin M, Ramm G, Demeler B, Dobson R

R_g^Guinier	3.3	nm
D_max	9.8	nm
Volume^Porod	108	nm³

SASDFW6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2)

80bp_DNA Forward80bp_DNA ReverseDNA-binding protein HU-alpha, E38K/V42L double mutant experimental SAS data

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	5.7	nm
D_max	31.3	nm
Volume^Porod	297	nm³

SASDFX6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	5.7	nm
D_max	25.7	nm
Volume^Porod	195	nm³

SASDFY6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant octamer, 76 kDa Escherichia coli protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	5.8	nm
D_max	28.1	nm
Volume^Porod	296	nm³

SASDFZ6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:16)

Sample:	80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant 16-mer, 153 kDa Linked to wild-type … protein
Buffer:	50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23

Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)
Soumya G Remesh

R_g^Guinier	6.3	nm
D_max	27.3	nm
Volume^Porod	401	nm³

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