MACROMOLECULE TYPE: heterocomplex

SASDUE5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 25mer of dsRNA in a 2:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 325-mer dsRNAInterleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
25-mer dsRNA monomer, 16 kDa RNA
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 5.7 nm
Dmax 19.9 nm
VolumePorod 453 nm3

SASDUF5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 25mer of dsRNA in a 4:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 325-mer dsRNAInterleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
25-mer dsRNA monomer, 16 kDa RNA
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 5.7 nm
Dmax 20.5 nm
VolumePorod 431 nm3

SASDUG5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 36mer of dsRNA in a 2:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 336-mer dsRNAInterleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
36-mer dsRNA monomer, 23 kDa RNA
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 6.1 nm
Dmax 21.2 nm
VolumePorod 587 nm3

SASDUH5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 36mer of dsRNA in a 4:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 336-mer dsRNAInterleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
36-mer dsRNA monomer, 23 kDa RNA
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 6.4 nm
Dmax 22.1 nm
VolumePorod 626 nm3

SASDUJ5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 54mer of dsRNA in a 2:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 354-mer dsRNA experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
54-mer dsRNA monomer, 35 kDa RNA
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 7.1 nm
Dmax 25.0 nm
VolumePorod 797 nm3

SASDUK5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 54mer of dsRNA in a 4:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 354-mer dsRNAInterleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
54-mer dsRNA monomer, 35 kDa RNA
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 7.7 nm
Dmax 26.5 nm
VolumePorod 1075 nm3

SASDUL5 – Interleukin enhancer-binding factor 3 (1-591) and Interleukin enhancer-binding factor 2 (1-390) heterodimer complex oligomerised along 54mer of dsRNA in a 6:1 ratio

Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2Interleukin enhancer-binding factor 354-mer dsRNAInterleukin enhancer-binding factor 3Interleukin enhancer-binding factor 2 experimental SAS data
DAMMIN model
Sample: Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
54-mer dsRNA monomer, 35 kDa RNA
Interleukin enhancer-binding factor 3 monomer, 66 kDa Mus musculus protein
Interleukin enhancer-binding factor 2 monomer, 44 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 May 9
Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA. Nucleic Acids Res 53(6) (2025)
Winterbourne S, Jayachandran U, Zou J, Rappsilber J, Granneman S, Cook AG
RgGuinier 7.8 nm
Dmax 26.1 nm
VolumePorod 1050 nm3

SASDUN4 – Signal transducer and activator of transcription 1-alpha/beta bound to transducer/activator DNA (STAT1/DNA)

Signal transducer and activator of transcription 1-alpha/betaSignal transducer and activator of transcription 1 binding DNA experimental SAS data
Signal transducer and activator of transcription 1-alpha/beta Signal transducer and activator of transcription 1 binding DNA Kratky plot
Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein
Signal transducer and activator of transcription 1 binding DNA dimer, 11 kDa Homo sapiens DNA
Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11
Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein Science Signaling 18(878) (2025)
Sugiyama A, Minami M, Ugajin K, Inaba-Inoue S, Yabuno N, Takekawa Y, Xiaomei S, Takei S, Sasaki M, Nomai T, Jiang X, Kita S, Maenaka K, Hirose M, Yao M, Gooley P, Moseley G, Sugita Y, Ose T
RgGuinier 6.1 nm
Dmax 19.4 nm
VolumePorod 761 nm3

SASDVU5 – Marchantia polymorpha Auxin Response Factor 3 in complex with high affinity DNA

Auxin response factorHigh Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing experimental SAS data
SWISSMODEL model
Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
High Affinity ARF binding sequence inverted repeat with 6 nucleotide spacing dimer, 12 kDa DNA
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 3.1 nm
Dmax 8.4 nm
VolumePorod 67 nm3

SASDVV5 – Arabidopsis thaliana Auxin Response Factor 1 in complex with high affinity DNA

Auxin response factor 1High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing experimental SAS data
Auxin response factor 1 High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing Kratky plot
Sample: Auxin response factor 1 dimer, 81 kDa Arabidopsis thaliana protein
High Affinity ARF binding sequence inverted repeat with 7 nucleotide spacing dimer, 13 kDa DNA
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 3.6 nm
Dmax 10.5 nm
VolumePorod 136 nm3