Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: nucleic acid

SASDRK4 – Diffusive GAC rRNA + Mg: Time-Resolved 10 ms

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA experimental SAS data

58 nucleotide RNA L11-binding domain from E. coli 23S rRNA Kratky plot

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDRL4 – Diffusive GAC rRNA + Mg: Time-Resolved 30 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDRM4 – Diffusive GAC rRNA + Mg: Time-Resolved 100 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	9.0	nm
Volume^Porod	29	nm³

SASDRN4 – Diffusive GAC rRNA + Mg: Time-Resolved 300 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.2	nm
D_max	12.0	nm
Volume^Porod	29	nm³

SASDRP4 – Diffusive GAC rRNA + Mg: Time-Resolved 1000 ms

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.4	nm
D_max	13.0	nm
Volume^Porod	30	nm³

SASDRQ4 – Diffusive GAC rRNA + Mg: Equilibrium initial

Sample:	58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
Buffer:	10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	34	nm³

SASDQ99 – Immunoglobulin G1 (pH 2, 0 M NaCl), SEC-SAXS data

humanized immunoglobulin G1 monoclonal antibody experimental SAS data

humanized immunoglobulin G1 monoclonal antibody Kratky plot

Sample:	Humanized immunoglobulin G1 monoclonal antibody monomer, 148 kDa Mouse/human (chimera)
Buffer:	100 mM glycine-HCl, pH: 2
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Mar 2

Getting Smaller by Denaturation: Acid-Induced Compaction of Antibodies The Journal of Physical Chemistry Letters :3898-3906 (2023)
Imamura H, Ooishi A, Honda S

R_g^Guinier	5.1	nm
D_max	17.6	nm
Volume^Porod	260	nm³

SASDQA9 – Immunoglobulin G1 (pH 2, 0.2 M NaCl), SEC-SAXS data

Sample:	Humanized immunoglobulin G1 monoclonal antibody monomer, 148 kDa Mouse/human (chimera)
Buffer:	100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment:	SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Mar 5

Getting Smaller by Denaturation: Acid-Induced Compaction of Antibodies The Journal of Physical Chemistry Letters :3898-3906 (2023)
Imamura H, Ooishi A, Honda S

R_g^Guinier	4.1	nm
D_max	14.2	nm
Volume^Porod	240	nm³

SASDQL9 – Japanese encephalitis virus 5' TR RNA

Japanese encephaltitis 5' TR experimental SAS data

Sample:	Japanese encephaltitis 5' TR monomer, 74 kDa Japanese encephalitis virus RNA
Buffer:	10 mM Bis-tris, 100 mM NaCl, 15 mM KCl 15 mM MgCl2, 10% glycerol, pH: 5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 22

Investigating RNA-RNA interactions through computational and biophysical analysis. Nucleic Acids Res (2023)
Mrozowich T, Park SM, Waldl M, Henrickson A, Tersteeg S, Nelson CR, De Klerk A, Demeler B, Hofacker IL, Wolfinger MT, Patel TR

R_g^Guinier	7.0	nm
D_max	22.4	nm
Volume^Porod	356	nm³

SASDQM9 – Japanese encephalitis virus 3' UTR RNA

Japanese encephalitis virus 3' UTR experimental SAS data

Sample:	Japanese encephalitis virus 3' UTR monomer, 186 kDa Japanese encephalitis virus RNA
Buffer:	10 mM Bis-tris, 100 mM NaCl, 15 mM KCl 15 mM MgCl2, 10% glycerol, pH: 5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 22

R_g^Guinier	11.3	nm
D_max	34.6	nm
Volume^Porod	2900	nm³

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