Browse by MACROMOLECULE type: nucleic acid

SASDDG5 – Mammalian prion protein mRNA (PrP mRNA wild type)

octo-repeat PrP mRNA experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Nov 4
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 7.4 nm
Dmax 25.0 nm
VolumePorod 165 nm3

SASDDH5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl

octo-repeat PrP mRNA experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 8.8 nm
Dmax 31.0 nm
VolumePorod 240 nm3

SASDDJ5 – Mammalian prion protein mRNA (PrP mRNA wild type) with LiCl

octo-repeat PrP mRNA experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM LiCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 9.0 nm
Dmax 31.0 nm
VolumePorod 225 nm3

SASDDK5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl and pyridostatin (PDS)

octo-repeat PrP mRNA experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 9.1 nm
Dmax 31.0 nm
VolumePorod 220 nm3

SASDDL5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA)

octo-repeat PrP mRNA mutant experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 7.0 nm
Dmax 24.0 nm
VolumePorod 150 nm3

SASDDM5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA) with KCl

octo-repeat PrP mRNA mutant experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM KCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 9.0 nm
Dmax 33.0 nm
VolumePorod 210 nm3

SASDDN5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with LiCl

octo-repeat PrP mRNA mutant experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM LiCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 8.9 nm
Dmax 33.0 nm
VolumePorod 223 nm3

SASDDP5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with KCl and pyridostatin (PDS)

octo-repeat PrP mRNA mutant experimental SAS data
DAMFILT model
Sample: Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer: 10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6
Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z
RgGuinier 9.2 nm
Dmax 33.0 nm
VolumePorod 260 nm3

SASDED8 – Poly-L-Glutamic Acid in Dimethyl Sulfoxide (DMSO)

Poly-L-Glutamic Acid experimental SAS data
PYMOL model
Sample: Poly-L-Glutamic Acid, 6 kDa
Buffer: Dimethyl Sulfoxide (DMSO), pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 11
β2-Type Amyloidlike Fibrils of Poly-l-glutamic Acid Convert into Long, Highly Ordered Helices upon Dissolution in Dimethyl Sulfoxide. J Phys Chem B 122(50):11895-11905 (2018)
Berbeć S, Dec R, Molodenskiy D, Wielgus-Kutrowska B, Johannessen C, Hernik-Magoń A, Tobias F, Bzowska A, Ścibisz G, Keiderling TA, Svergun D, Dzwolak W
RgGuinier 1.8 nm
Dmax 7.8 nm
VolumePorod 5 nm3

SASDC74 – Truncated P5abc subdomain from tetrahymena ribozyme: Time-resolved 0ms

Truncated P5abc subdomain from tetrahymena ribozyme experimental SAS data
Truncated P5abc subdomain from tetrahymena ribozyme Kratky plot
Sample: Truncated P5abc subdomain from tetrahymena ribozyme monomer, 18 kDa RNA
Buffer: 10mM KMOPS 20mM KCl 1mM MgCl2 20uM EDTA, pH: 7
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 3
Revealing the distinct folding phases of an RNA three-helix junction. Nucleic Acids Res 46(14):7354-7365 (2018)
Plumridge A, Katz AM, Calvey GD, Elber R, Kirmizialtin S, Pollack L
RgGuinier 2.5 nm
Dmax 8.0 nm