Browse by MACROMOLECULE type: protein

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.0 nm
VolumePorod 98 nm3

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 4.0 nm
Dmax 13.2 nm
VolumePorod 211 nm3

SASDFS8 – Yeast alcohol dehydrogenase 1 - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Alcohol dehydrogenase 1 experimental SAS data
DAMMIN model
Sample: Alcohol dehydrogenase 1 tetramer, 147 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 3.3 nm
Dmax 9.3 nm
VolumePorod 201 nm3

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Bovine serum albumin experimental SAS data
GASBOR model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23
Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C
RgGuinier 2.8 nm
Dmax 8.3 nm
VolumePorod 95 nm3

SASDJ43 – Acyl carrier protein-thioesterase domain from Men2

Menisporopsin singlet acyl carrier protein-thioesterase experimental SAS data
Acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin singlet acyl carrier protein-thioesterase monomer, 45 kDa Menisporopsin theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
Bunnak W, Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 3.6 nm
Dmax 14.4 nm
VolumePorod 75 nm3

SASDJ53 – Doublet acyl carrier protein-thioesterase domain from Men2

Menisporopsin doublet Acyl Carrier Protein-Thioesterase experimental SAS data
Doublet acyl carrier protein-thioesterase domain from Men2 Rg histogram
Sample: Menisporopsin doublet Acyl Carrier Protein-Thioesterase monomer, 58 kDa Menisporopsis theobromae BCC … protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2020 Jan 25
SAXS reveals highly flexible interdomain linkers of tandem acyl carrier protein–thioesterase domains from a fungal nonreducing polyketide synthase FEBS Letters (2020)
Bunnak W, Winter A, Lazarus C, Crump M, Race P, Wattana‐Amorn P
RgGuinier 4.2 nm
Dmax 17.3 nm
VolumePorod 84 nm3

SASDJ92 – S. epidermidis extracellular matrix binding protein (Embp) F-repeats

Extracellular matrix binding protein F-repeats experimental SAS data
SASREF model
Sample: Extracellular matrix binding protein F-repeats, 70 kDa Staphylococcus epidermidis protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 15
A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism. mBio 11(5) (2020)
Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H
RgGuinier 7.0 nm
Dmax 28.0 nm

SASDJA2 – S. epidermidis extracellular matrix binding protein (Embp) FG-repeats

Extracellular matrix binding protein FG-repeats experimental SAS data
EOM/RANCH model
Sample: Extracellular matrix binding protein FG-repeats, 85 kDa Staphylococcus epidermidis protein
Buffer: PBS, 136.5 mM NaCl, 2.65 mM KCl, 8.3 mM Na2HPO4, 2.65 mM KH2PO4, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
A Giant Extracellular Matrix Binding Protein of Staphylococcus epidermidis Binds Surface-Immobilized Fibronectin via a Novel Mechanism. mBio 11(5) (2020)
Büttner H, Perbandt M, Kohler T, Kikhney A, Wolters M, Christner M, Heise M, Wilde J, Weißelberg S, Both A, Betzel C, Hammerschmidt S, Svergun D, Aepfelbacher M, Rohde H
RgGuinier 10.7 nm
Dmax 40.0 nm

SASDHJ8 – ACT domain of the Mycobacterium tuberculosis Rel protein

ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) experimental SAS data
ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) Kratky plot
Sample: ACT domain of Rel protein (Bifunctional (p)ppGpp synthase/hydrolase RelA) dimer, 20 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 350 mM NaCl, 5% glycerol, 1 mM DTT, pH: 8.5
Experiment: SAXS data collected at Bruker Nanostar, Nanyang Technological University on 2018 Jun 7
Atomic structure of, and valine binding to the regulatory ACT domain of the Mycobacterium tuberculosis Rel protein. FEBS J (2020)
Shin J, Singal B, Manimekalai MSS, Chen MW, Ragunathan P, Grüber G
RgGuinier 1.9 nm
Dmax 6.1 nm
VolumePorod 29 nm3

SASDH64 – Plasmodium falciparum myosin essential light chain, N-terminal domain

Myosin essential light chain experimental SAS data
PYMOL model
Sample: Myosin essential light chain monomer, 9 kDa Plasmodium falciparum protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Oct 25
Structural role of essential light chains in the apicomplexan glideosome. Commun Biol 3(1):568 (2020)
Pazicky S, Dhamotharan K, Kaszuba K, Mertens HDT, Gilberger T, Svergun D, Kosinski J, Weininger U, Löw C
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 12 nm3