Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDHD7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1089 Gy X-ray dose (J/kg)

Endo-beta-N-acetylglucosaminidase H experimental SAS data

Endo-beta-N-acetylglucosaminidase H Kratky plot

Sample:	Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4

SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T

R_g^Guinier	2.7	nm
Volume^Porod	60	nm³

SASDHE7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1125.3 Gy X-ray dose (J/kg)

Sample:	Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4

SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T

R_g^Guinier	2.7	nm
Volume^Porod	61	nm³

SASDHF7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1161.6 Gy X-ray dose (J/kg)

Sample:	Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4

SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T

R_g^Guinier	2.8	nm
Volume^Porod	61	nm³

SASDHG7 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 1197.9 Gy X-ray dose (J/kg)

Sample:	Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer:	20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4

SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T

R_g^Guinier	2.7	nm
Volume^Porod	60	nm³

SASDJB5 – Nipah virus phosphoprotein, N-terminal amino acids 1-406 (PNT)

Nipah virus phosphoprotein, N-terminal amino acids 1-406 (PNT) Rg histogram

Sample:	Phosphoprotein monomer, 45 kDa Nipah henipavirus protein
Buffer:	20 mM Tris-HCl, 0.3 M NaCl, 5 mM DTT, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2018 May 3

Ensemble description of the intrinsically disordered N-terminal domain of the Nipah virus P/V protein from combined NMR and SAXS. Sci Rep 10(1):19574 (2020)
Schiavina M, Salladini E, Murrali MG, Tria G, Felli IC, Pierattelli R, Longhi S

R_g^Guinier	6.2	nm
D_max	23.0	nm
Volume^Porod	210	nm³

SASDJF4 – Synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23 experimental SAS data

Sample:	Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein
Buffer:	50 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 5

Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 Nature Communications 11(1) (2020)
Custódio T, Das H, Sheward D, Hanke L, Pazicky S, Pieprzyk J, Sorgenfrei M, Schroer M, Gruzinov A, Jeffries C, Graewert M, Svergun D, Dobrev N, Remans K, Seeger M, McInerney G, Murrell B, Hällberg B, Löw C

R_g^Guinier	2.1	nm
D_max	8.0	nm
Volume^Porod	22	nm³

SASDJG4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD)

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 1

R_g^Guinier	3.0	nm
D_max	13.1	nm
Volume^Porod	64	nm³

SASDJH4 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) bound to the synthetic nanobody Sybody 23 (Sy23)

Synthetic nanobody Sybody 23Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Sample:	Synthetic nanobody Sybody 23 monomer, 16 kDa synthetic construct protein Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	25 mM Tris 100 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 May 10

R_g^Guinier	3.5	nm
D_max	15.1	nm
Volume^Porod	87	nm³

SASDFN8 – Apoferritin from horse spleen - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Apoferritin light chain experimental SAS data

Sample:	Apoferritin light chain 24-mer, 479 kDa Equus caballus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C

R_g^Guinier	5.4	nm
D_max	12.5	nm
Volume^Porod	679	nm³

SASDFP8 – Carbonic anhydrase 2 from bovine erythrocytes - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

Carbonic anhydrase 2 experimental SAS data

Sample:	Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	1.8	nm
D_max	5.1	nm
Volume^Porod	37	nm³

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