Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDH86 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within High Five insect cells

Cathepsin B-like cysteine protease experimental SAS data

Cathepsin B-like cysteine protease Kratky plot

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDH96 – In cellulo cathepsin B (CatB) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Cathepsin B-like cysteine protease, 37 kDa Trypanosoma brucei protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Sample:	Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase, 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

Myotilin (222-452) experimental SAS data

Sample:	Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	36	nm³

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

Myotilin (222-452) R405K experimental SAS data

Sample:	Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	36	nm³

SASDHP5 – Rov C - Regulator Of Virulence interconnected with the Csr system (hexameric transcriptional activator of type VI secretion gene expression)

Regulator Of Virulence interconnected with the Csr system experimental SAS data

Sample:	Regulator Of Virulence interconnected with the Csr system hexamer, 175 kDa Yersinia pseudotuberculosis protein
Buffer:	50 mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 18

RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression PLOS Pathogens 16(9):e1008552 (2020)
Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J

R_g^Guinier	4.2	nm
D_max	12.4	nm
Volume^Porod	280	nm³

SASDHC6 – C-terminal domain-like carotenoid protein (CCP2), apo-dimer

C-terminal domain-like carotenoid protein experimental SAS data

Sample:	C-terminal domain-like carotenoid protein dimer, 33 kDa Tolypothrix sp. PCC … protein
Buffer:	10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15

Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
Dominguez-Martin M, Hammel M, Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C

R_g^Guinier	2.5	nm
D_max	9.1	nm
Volume^Porod	55	nm³

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