Browse by MACROMOLECULE type: protein

SASDF77 – Kinase-inducible domain interacting (KIX) domain of CREB-binding protein tethered to 1-10

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C1-{4-[4-chloro-3-(trifluoromethyl)phenyl]-4-hydroxypiperidin-1-yl}-3-sulfanylpropan-1-one experimental SAS data
DAMMIN model
Sample: Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein
1-{4-[4-chloro-3-(trifluoromethyl)phenyl]-4-hydroxypiperidin-1-yl}-3-sulfanylpropan-1-one monomer, 0 kDa
Buffer: 25 mM Hepes, 150 NaCl, 5% Glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27
Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. J Biol Chem (2019)
Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E
RgGuinier 1.7 nm
Dmax 6.4 nm

SASDGL2 – Ring opening PaaZ from the phenylacetate degradation pathway (E. coli K12)

Bifunctional protein PaaZ experimental SAS data
OTHER model
Sample: Bifunctional protein PaaZ hexamer, 438 kDa Escherichia coli protein
Buffer: 25 mM HEPES, 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Feb 24
Molecular basis for metabolite channeling in a ring opening enzyme of the phenylacetate degradation pathway. Nat Commun 10(1):4127 (2019)
Sathyanarayanan N, Cannone G, Gakhar L, Katagihallimath N, Sowdhamini R, Ramaswamy S, Vinothkumar KR
RgGuinier 6.2 nm
Dmax 20.0 nm
VolumePorod 636 nm3

SASDC68 – Surface presentation of antigens protein, SpaOc

Surface presentation of antigens protein SpaO SpaO(SPOA2) experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.5 nm
Dmax 9.1 nm
VolumePorod 51 nm3

SASDC78 – Surface presentation of antigens protein SpaOc-SpaO(SPOA1,2)

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2) experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 3.3 nm
Dmax 11.1 nm
VolumePorod 108 nm3

SASDC88 – Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus

Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus experimental SAS data
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 1.6 nm
Dmax 5.2 nm
VolumePorod 31 nm3

SASDC98 – Surface presentation of antigens protein SpaOc-SpaO N-terminus

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) N-terminus monomer, 17 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.9 nm
Dmax 10.6 nm
VolumePorod 68 nm3

SASDEJ7 – The InvC-ATP synthase, surface presentation of antigens and oxygen-regulated invasion protein (ISO) complex

Surface presentation of antigens protein SpaO SpaO(SPOA2)Surface presentation of antigens protein SpaO(SPOA1,2)Oxygen-regulated invasion protein OrgBATP synthase  InvC experimental SAS data
Surface presentation of antigens protein SpaO SpaO(SPOA2) Surface presentation of antigens protein SpaO(SPOA1,2) Oxygen-regulated invasion protein OrgB ATP synthase  InvC Kratky plot
Sample: Surface presentation of antigens protein SpaO SpaO(SPOA2) dimer, 25 kDa Salmonella enterica subsp. … protein
Surface presentation of antigens protein SpaO(SPOA1,2) monomer, 34 kDa Salmonella enterica subsp. … protein
Oxygen-regulated invasion protein OrgB dimer, 53 kDa Salmonella enterica subsp. … protein
ATP synthase InvC monomer, 48 kDa Salmonella enterica subsp. … protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 12
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 6.0 nm
Dmax 22.7 nm
VolumePorod 302 nm3

SASDEK7 – SpaO C-terminus

Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus experimental SAS data
Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus Kratky plot
Sample: Surface presentation of antigens protein SpaO(SPOA1,2) C-terminus monomer, 19 kDa Salmonella enterica subsp. … protein
Buffer: 20 mM HEPES pH 7.5, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 24
Molecular Organization of Soluble Type III Secretion System Sorting Platform Complexes. J Mol Biol 431(19):3787-3803 (2019)
Bernal I, Börnicke J, Heidemann J, Svergun D, Horstmann JA, Erhardt M, Tuukkanen A, Uetrecht C, Kolbe M
RgGuinier 2.1 nm
Dmax 7.2 nm

SASDFD7 – Pseudomonas putida CBB5 NdmAB complex - static

Methylxanthine N1-demethylase NdmAMethylxanthine N3-demethylase NdmB experimental SAS data
DAMMIF model
Sample: Methylxanthine N1-demethylase NdmA trimer, 127 kDa Pseudomonas putida protein
Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein
Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27
Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK
RgGuinier 4.5 nm
Dmax 12.3 nm

SASDFE7 – Pseudomonas putida CBB5 mCherry-NdmA/NdmB complex - static

Methylxanthine N1-demethylase NdmAMethylxanthine N3-demethylase NdmB experimental SAS data
DAMMIF model
Sample: Methylxanthine N1-demethylase NdmA trimer, 207 kDa Pseudomonas putida protein
Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein
Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27
Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex. J Mol Biol 431(19):3647-3661 (2019)
Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK
RgGuinier 5.4 nm
Dmax 13.8 nm