Browse by MACROMOLECULE type: protein

SASDGP2 – Thymine dioxygenase full length J-DNA binding protein (JBP1)

Thymine dioxygenase JBP1 experimental SAS data
Thymine dioxygenase JBP1 Kratky plot
Sample: Thymine dioxygenase JBP1 monomer, 93 kDa Leishmania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 3.4 nm
Dmax 12.0 nm
VolumePorod 128 nm3

SASDGQ2 – Thymine dioxygenase lacking the JDNA binding domain Delta-JDBD (Δ-JDBD)

Delta-JDBD experimental SAS data
Delta-JDBD Kratky plot
Sample: Delta-JDBD monomer, 72 kDa Leismania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 21
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 3.1 nm
Dmax 9.9 nm
VolumePorod 108 nm3

SASDGR2 – Thymine dioxygenase J-containing DNA binding domain (JDBD)

J-DNA binding domain experimental SAS data
J-DNA binding domain Kratky plot
Sample: J-DNA binding domain monomer, 21 kDa Leishmania tarentolae protein
Buffer: 20 mM HEPES, 200 mM NaCl, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 4
The domain architecture of protozoan protein J-DNA-binding protein 1 suggests synergy between base J DNA binding and thymidine hydroxylase activity. J Biol Chem (2019)
Adamopoulos A, Heidebrecht T, Roosendaal J, Touw WG, Phan IQ, Beijnen J, Perrakis A
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 38 nm3

SASDED9 – Relaxase domain of TraI

Relaxase (Tra_2) domain of TraI experimental SAS data
GASBOR model
Sample: Relaxase (Tra_2) domain of TraI monomer, 46 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.6 nm
Dmax 8.3 nm
VolumePorod 61 nm3

SASDEE9 – TraI_2_C domain of TraI

TraI_2_C domain of TraI experimental SAS data
GASBOR model
Sample: TraI_2_C domain of TraI monomer, 21 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Jul 11
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 2.2 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDEF9 – TraI of Neisseria gonorrhoeae

TraI experimental SAS data
GASBOR model
Sample: TraI monomer, 91 kDa Neisseria gonorrhoeae protein
Buffer: 50 mM TRIS-HCl 100 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 5
DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island. Nucleic Acids Res 47(15):8136-8153 (2019)
Heilers JH, Reiners J, Heller EM, Golzer A, Smits SHJ, van der Does C
RgGuinier 7.3 nm
Dmax 31.4 nm
VolumePorod 293 nm3

SASDFD2 – wild-type human Latent Transforming Growth Factor beta 1 (LTGFB-1)

Human Latent Transforming Growth Factor beta 1 experimental SAS data
Human Latent Transforming Growth Factor beta 1 Kratky plot
Sample: Human Latent Transforming Growth Factor beta 1 dimer, 86 kDa Homo sapiens protein
Buffer: phosphate buffered saline 2% glycerol, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 4
Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses. J Synchrotron Radiat 26(Pt 4):967-979 (2019)
Stachowski T, Grant TD, Snell EH
RgGuinier 3.8 nm
Dmax 17.5 nm
VolumePorod 200 nm3

SASDFE2 – wild-type human Latency Associated Peptide (LAP)

Latency Associated Peptide experimental SAS data
Latency Associated Peptide Kratky plot
Sample: Latency Associated Peptide dimer, 58 kDa Homo sapiens protein
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Oct 4
Structural consequences of transforming growth factor beta-1 activation from near-therapeutic X-ray doses. J Synchrotron Radiat 26(Pt 4):967-979 (2019)
Stachowski T, Grant TD, Snell EH
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 179 nm3

SASDF93 – Ignicoccus islandicus malate dehydrogenase

Malate dehydrogenase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Malate dehydrogenase tetramer, 134 kDa Ignicoccus islandicus DSM … protein
Buffer: 50 mM Tris-HCl 50 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Sep 5
The archaeal LDH-like malate dehydrogenase from Ignicoccus islandicus displays dual substrate recognition, hidden allostery and a non-canonical tetrameric oligomeric organization Journal of Structural Biology (2019)
Roche J, Girard E, Mas C, Madern D
RgGuinier 3.3 nm
Dmax 9.0 nm
VolumePorod 198 nm3

SASDF94 – Insulin glulisine (Apidra), oligomeric composition

Insulin glulisine experimental SAS data
CUSTOM IN-HOUSE model
Sample: Insulin glulisine hexamer, 35 kDa protein
Buffer: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20
The quaternary structure of insulin glargine and glulisine under formulation conditions. Biophys Chem 253:106226 (2019)
Nagel N, Graewert MA, Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H
RgGuinier 2.3 nm
Dmax 7.6 nm