Browse by MACROMOLECULE type: protein

SASDA49 – Cation-free slp-B53

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
Liu J, Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 5.8 nm
Dmax 22.0 nm
VolumePorod 495 nm3

SASDA59 – slp-B53 with Ca2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Ca2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
Liu J, Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.4 nm
Dmax 28.1 nm
VolumePorod 609 nm3

SASDA69 – slp-B53 with Mg2+

S-layer protein experimental SAS data
DAMMIF model
Sample: S-layer protein monomer, 116 kDa Lysinibacillus sphaericus protein
Buffer: Water with Mg2+, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus. Eur Biophys J 46(1):77-89 (2017)
Liu J, Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J
RgGuinier 6.6 nm
Dmax 29.0 nm
VolumePorod 597 nm3

SASDBW4 – Albumen gland precursor of ovorubin (lipoglycocarotenoprotein agPcOvo: an oligomeric protein)

Perivitellin ovorubin-1Perivitellin ovorubin-2Perivitellin ovorubin-3 experimental SAS data
DAMMIF model
Sample: Perivitellin ovorubin-1, 22 kDa Pomacea canaliculata protein
Perivitellin ovorubin-2, 24 kDa Pomacea canaliculata protein
Perivitellin ovorubin-3, 35 kDa Pomacea canaliculata protein
Buffer: 20 mM Tris-HCl, pH: 8.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2014 Jun 10
Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior. Physiol Biochem Zool 90(4):461-470 (2017)
Cadierno MP, Dreon MS, Heras H
RgGuinier 4.3 nm
Dmax 14.9 nm
VolumePorod 526 nm3

SASDBX4 – Albumen gland precursor of perivitellin-2 (PcPV2)

Perivitellin-2 31 kDa subunitPerivitellin-2 67 kDa subunit experimental SAS data
DAMMIF model
Sample: Perivitellin-2 31 kDa subunit tetramer, 126 kDa Pomacea canaliculata protein
Perivitellin-2 67 kDa subunit tetramer, 250 kDa Pomacea canaliculata protein
Buffer: 20 mM Tris-HCl, pH: 8.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2014 Jun 10
Apple Snail Perivitellin Precursor Properties Help Explain Predators' Feeding Behavior. Physiol Biochem Zool 90(4):461-470 (2017)
Cadierno MP, Dreon MS, Heras H
RgGuinier 4.8 nm
Dmax 17.0 nm
VolumePorod 294 nm3

SASDBY6 – Leishmania braziliensis Mitochondrial heat shock protein 70 (LbmtHSP70)

Mitochondrial heat shock protein 70 experimental SAS data
DAMMIN model
Sample: Mitochondrial heat shock protein 70 monomer, 71 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris-HCl, 50 mM NacL, 5 mM KCl, 5 mM sodium phosphate, 2 mM B-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2012 Jun 22
Structural and functional studies of the Leishmania braziliensis mitochondrial Hsp70: Similarities and dissimilarities to human orthologues. Arch Biochem Biophys 613:43-52 (2017)
Dores-Silva PR, Nishimura LS, Kiraly VT, Borges JC
RgGuinier 3.6 nm
Dmax 14.0 nm
VolumePorod 118 nm3

SASDC58 – Dipeptidyl peptidase III: pgDPP3_FL

Peptidase, M49 family experimental SAS data
DAMMIN model
Sample: Peptidase, M49 family monomer, 102 kDa Porphyromonas gingivalis protein
Buffer: 50mM Tris,100mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 14
A novel Porphyromonas gingivalis enzyme: An atypical dipeptidyl peptidase III with an ARM repeat domain. PLoS One 12(11):e0188915 (2017)
Hromić-Jahjefendić A, Jajčanin Jozić N, Kazazić S, Grabar Branilović M, Karačić Z, Schrittwieser JH, Das KMP, Tomin M, Oberer M, Gruber K, Abramić M, Tomić S
RgGuinier 3.0 nm
Dmax 11.3 nm
VolumePorod 116 nm3

SASDCM8 – Mo-insertase domain Cnx1E from Arabidopsis thaliana

Molybdopterin biosynthesis protein CNX1 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Molybdopterin biosynthesis protein CNX1 dimer, 99 kDa Arabidopsis thaliana protein
Buffer: 20 mM Hepes/KOH, 150 mM NaCl, 1 mM EDTA, 2 % (v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at ID14-3, ESRF on 2015 Feb 5
Dimerization of the plant molybdenum insertase Cnx1E is required for synthesis of the molybdenum cofactor. Biochem J 474(1):163-178 (2017)
Krausze J, Probst C, Curth U, Reichelt J, Saha S, Schafflick D, Heinz DW, Mendel RR, Kruse T
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 181 nm3

SASDB55 – Glycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)

Myelin-associated glycoprotein Ig domains 1-5 experimental SAS data
NONE model
Sample: Myelin-associated glycoprotein Ig domains 1-5 dimer, 108 kDa Mus musculus protein
Buffer: 25 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 6.8 nm
Dmax 23.8 nm
VolumePorod 177 nm3

SASDB26 – Glycosylated myelin-associated glycoprotein full extracellular domain (Ig1-5) I473E mutant

Myelin-associated glycoprotein (20-508; I473E mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myelin-associated glycoprotein (20-508; I473E mutant) monomer, 54 kDa Mus musculus protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 6.0 nm
Dmax 20.6 nm
VolumePorod 117 nm3