Browse by MACROMOLECULE type: protein

SASDBS2 – Recombinant Tn antigen-binding lectin from Vatairea macrocarpa

Recombinant Tn antigen-binding lectin experimental SAS data
CORAL model
Sample: Recombinant Tn antigen-binding lectin tetramer, 105 kDa Vatairea macrocarpa protein
Buffer: 100 mM sodium phosphate 150 mM NaCl 5% (v/v) glycerol, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 11
Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: A new tool for cancer research. Int J Biochem Cell Biol 72:27-39 (2016)
Sousa BL, Silva-Filho JC, Kumar P, Graewert MA, Pereira RI, Cunha RMS, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS
RgGuinier 3.2 nm
Dmax 9.5 nm
VolumePorod 168 nm3

SASDBG3 – Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct)

Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) experimental SAS data
DAMMIN model
Sample: Mouse Leucine-rich repeat transmembrane neuronal protein 2, cLRRTM2 (stability engineered construct) monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris 150 mM NaCl 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ID14-3, ESRF on 2015 Sep 27
Crystal Structure of an Engineered LRRTM2 Synaptic Adhesion Molecule and a Model for Neurexin Binding. Biochemistry 55(6):914-26 (2016)
Paatero A, Rosti K, Shkumatov AV, Sele C, Brunello C, Kysenius K, Singha P, Jokinen V, Huttunen H, Kajander T
RgGuinier 3.3 nm
Dmax 13.1 nm

SASDBH3 – Leucine-rich repeat transmembrane neuronal protein 2, LRRTM2 (fragment 30-380)

Mouse Leucine-rich repeat transmembrane neuronal protein 2, LRRTM2 experimental SAS data
DAMMIN model
Sample: Mouse Leucine-rich repeat transmembrane neuronal protein 2, LRRTM2 dimer, 80 kDa Mus musculus protein
Buffer: 20 mM Tris 150 mM NaCl 3% glycerol, pH: 7.4
Experiment: SAXS data collected at ID14-3, ESRF on 2015 Jun 28
Crystal Structure of an Engineered LRRTM2 Synaptic Adhesion Molecule and a Model for Neurexin Binding. Biochemistry 55(6):914-26 (2016)
Paatero A, Rosti K, Shkumatov AV, Sele C, Brunello C, Kysenius K, Singha P, Jokinen V, Huttunen H, Kajander T
RgGuinier 4.2 nm
Dmax 21.6 nm

SASDBC3 – RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158

Replication initiator protein of a promiscuous streptococcal plasmid pMV158. experimental SAS data
NONE model
Sample: Replication initiator protein of a promiscuous streptococcal plasmid pMV158. hexamer, Streptococcus sp. protein
Buffer: 10 mM TRIS 5 mM EDTA 1.0 M KCl, pH: 8.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jun 16
Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158. Sci Rep 6:20915 (2016)
Boer DR, Ruiz-Masó JA, Rueda M, Petoukhov MV, Machón C, Svergun DI, Orozco M, del Solar G, Coll M
RgGuinier 3.8 nm
Dmax 10.6 nm
VolumePorod 282 nm3

SASDBT2 – Ankyrin repeat domains from human Tankyrase-2 (489-649)

Ankyrin repeat domains from Tankyrase 2 experimental SAS data
Ankyrin repeat domains from Tankyrase 2 Kratky plot
Sample: Ankyrin repeat domains from Tankyrase 2 monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 1.8 nm
Dmax 6.3 nm
VolumePorod 24 nm3

SASDBU2 – Human Arpin (isoform 1)

Human Arpin experimental SAS data
Human Arpin Kratky plot
Sample: Human Arpin monomer, 25 kDa Homo sapiens protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.6 nm
Dmax 13.2 nm
VolumePorod 47 nm3

SASDBV2 – Zebrafish Arpin

Zebrafish (Danio rerio) Arpin experimental SAS data
Zebrafish (Danio rerio) Arpin Kratky plot
Sample: Zebrafish (Danio rerio) Arpin monomer, 25 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.7 nm
Dmax 13.8 nm
VolumePorod 47 nm3

SASDBW2 – Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16).

Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). experimental SAS data
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Kratky plot
Sample: Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). monomer, 24 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.2 nm
Dmax 11.3 nm
VolumePorod 37 nm3

SASDBX2 – Zebrafish Arpin in complex with the ankyrin repeat domains of human Tankyrase 2 (489-469)

Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex experimental SAS data
Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex Kratky plot
Sample: Zebrafish arpin/human tankyrase 2 ankyrin repeat domain complex monomer, 43 kDa Danio rerio / … protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 3.3 nm
Dmax 12.6 nm
VolumePorod 58 nm3

SASDBQ3 – Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164)

Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) experimental SAS data
DAMMIF model
Sample: Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) monomer, 18 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Oct 5
Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering. Acta Crystallogr D Struct Biol 72(Pt 2):192-202 (2016)
Papageorgiou N, Lichière J, Baklouti A, Ferron F, Sévajol M, Canard B, Coutard B
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3