Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDSW9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 72.7 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data

Nucleoprotein (L223P, L227P, L230P) Kratky plot

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN

R_g^Guinier	3.5	nm
D_max	14.5	nm
Volume^Porod	47	nm³

SASDSX9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 294 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

R_g^Guinier	4.4	nm
D_max	19.0	nm
Volume^Porod	68	nm³

SASDSY9 – SARS-CoV-2 N-protein (N1-245, residues 1-245) L223P, L227P and L230P triple mutant: 400 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 27 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 Jan 29

R_g^Guinier	4.6	nm
D_max	19.0	nm
Volume^Porod	75	nm³

SASDSZ9 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 238 µM

Sample:	Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	19	nm³

SASDT22 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 476 µM

Sample:	Nucleoprotein monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	3.7	nm
D_max	16.0	nm
Volume^Porod	33	nm³

SASDT32 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245): 952 µM

Sample:	Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	4.1	nm
D_max	18.0	nm
Volume^Porod	53	nm³

SASDT42 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 238 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	2.4	nm
D_max	11.0	nm
Volume^Porod	10	nm³

SASDT52 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 476 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	2.7	nm
D_max	11.0	nm
Volume^Porod	12	nm³

SASDT62 – SARS-CoV-2 N-protein InterDomain Linker (IDL, residues 176-245) L223P, L227P and L230P triple mutant: 952 µM

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	2.7	nm
D_max	12.0	nm
Volume^Porod	13	nm³

SASDTE5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 26 µM

Sample:	Nucleoprotein dimer, 82 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	5.7	nm
D_max	23.0	nm
Volume^Porod	160	nm³

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