Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDTF5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 49 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

Dynamic ensembles of SARS-CoV-2 N-protein reveal head-to-head coiled-coil-driven oligomerization and phase separation. Nucleic Acids Res 53(11) (2025)
Hernandez G, Martins ML, Fernandes NP, Veloso T, Lopes J, Gomes T, Cordeiro TN

R_g^Guinier	5.8	nm
D_max	24.0	nm
Volume^Porod	181	nm³

SASDTG5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 103 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	6.2	nm
D_max	25.0	nm
Volume^Porod	215	nm³

SASDTH5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 206 µM

Sample:	Nucleoprotein tetramer, 163 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	6.8	nm
D_max	28.0	nm
Volume^Porod	578	nm³

SASDTJ5 – SARS-CoV-2 N-protein (N1-365, amino acids 1-365): 299 µM

Sample:	Nucleoprotein hexamer, 245 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	7.1	nm
D_max	29.0	nm
Volume^Porod	708	nm³

SASDTX5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 19 µM

Sample:	Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	6.3	nm
D_max	26.0	nm
Volume^Porod	233	nm³

SASDTY5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 50 µM

Sample:	Nucleoprotein tetramer, 187 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	6.9	nm
D_max	28.5	nm
Volume^Porod	571	nm³

SASDTZ5 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 103 µM

Sample:	Nucleoprotein hexamer, 281 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	7.6	nm
D_max	32.0	nm
Volume^Porod	764	nm³

SASDT26 – SARS-CoV-2 N-protein Full Length (N1-419, amino acids 1-419): 216 µM

Sample:	Nucleoprotein octamer, 375 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2022 May 13

R_g^Guinier	8.2	nm
D_max	33.0	nm
Volume^Porod	1028	nm³

SASDVS6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245): 1666.7 µM

Sample:	Nucleoprotein dimer, 17 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	4.5	nm
D_max	19.0	nm
Volume^Porod	117	nm³

SASDVT6 – SARS-CoV-2 N-protein InterDomain Linker (IDL, amino acids 176-245) L223P, L227P and L230P triple mutant: 1667 µM

Nucleoprotein (L223P, L227P, L230P) experimental SAS data

Nucleoprotein (L223P, L227P, L230P) Kratky plot

Sample:	Nucleoprotein (L223P, L227P, L230P) monomer, 8 kDa Severe acute respiratory … protein
Buffer:	100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2023 Jan 29

R_g^Guinier	2.8	nm
D_max	12.0	nm
Volume^Porod	14	nm³

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