Browse by MACROMOLECULE type: protein

SASDQ86 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 368 nm3

SASDQ96 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 5.5 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDQA6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) M1+M2 mutant L319A, S320A, L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: L319A, S320A, L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 15.5 nm
VolumePorod 352 nm3

SASDQB6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362) deletion mutant ΔE331-A341

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362: ΔE331-A341) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362: ΔE331-A341) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-362: ΔE331-A341) monomer, 6 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.2 nm
Dmax 28.0 nm
VolumePorod 912 nm3

SASDQC6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465)

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465) experimental SAS data
CORAL model
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 7.4 nm
Dmax 28.0 nm
VolumePorod 978 nm3

SASDQD6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 18.5 nm
VolumePorod 316 nm3

SASDQE6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 4.8 nm
Dmax 18.0 nm
VolumePorod 310 nm3

SASDQF6 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-465) M1+M2 mutant L319A, S320A, L345A, S346A

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) experimental SAS data
C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) Kratky plot
Sample: C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Retinoic acid-induced protein 2 (303-465: L319A, S320A, L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer: 50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13
Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M
RgGuinier 5.1 nm
Dmax 17.5 nm
VolumePorod 387 nm3

SASDUW6 – Alarmin release inhibitor (HpARI2), full-length

Alarmin release inhibitor (Δ1-62) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-62) monomer, 26 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.6 nm
Dmax 9.9 nm
VolumePorod 31 nm3

SASDUX6 – Alarmin release inhibitor (HpARI2) domains CCP2 and CCP3

Alarmin release inhibitor (Δ1-125; N175Q, N190Q) experimental SAS data
DAMMIF model
Sample: Alarmin release inhibitor (Δ1-125; N175Q, N190Q) monomer, 19 kDa Heligmosomoides polygyrus protein
Buffer: 137 mM NaCl, 2.7 mM KCl, 10 mM phosphate buffer, 5% glycerol, pH: 7.2
Experiment: SAXS data collected at B21, Diamond Light Source on 2024 Feb 9
Structural basis for IL-33 recognition and its antagonism by the helminth effector protein HpARI2. Nat Commun 15(1):5226 (2024)
Jamwal A, Colomb F, McSorley HJ, Higgins MK
RgGuinier 2.3 nm
Dmax 8.2 nm
VolumePorod 28 nm3