Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDQW5 – C-terminal-binding protein 1 (CtBP1)

C-terminal-binding protein 1 experimental SAS data

Sample:	C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

Master corepressor inactivation through multivalent SLiM-induced polymerization mediated by the oncogene suppressor RAI2. Nat Commun 15(1):5241 (2024)
Goradia N, Werner S, Mullapudi E, Greimeier S, Bergmann L, Lang A, Mertens H, Węglarz A, Sander S, Chojnowski G, Wikman H, Ohlenschläger O, von Amsberg G, Pantel K, Wilmanns M

R_g^Guinier	4.3	nm
D_max	14.0	nm
Volume^Porod	279	nm³

SASDQX5 – C-terminal binding protein 1 mutant (CtBP1: C134Y, N138R, R141E, L150W)

C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W) experimental SAS data

Sample:	C-terminal-binding protein 1 (C134Y, N138R, R141E, L150W), 48 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	3.4	nm
D_max	12.0	nm
Volume^Porod	154	nm³

SASDQY5 – C-terminal binding protein 1 mutant (CtBP1: R266A, D290A, E295A, H315A)

C-terminal-binding protein 1 (R266A, D290A, E295A, H315A) experimental SAS data

Sample:	C-terminal-binding protein 1 (R266A, D290A, E295A, H315A), 47 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	4.2	nm
D_max	15.0	nm
Volume^Porod	161	nm³

SASDQZ5 – Retinoic acid-induced protein 2 (RAI2: 303-362)

Retinoic acid-induced protein 2 (303-362) experimental SAS data

Retinoic acid-induced protein 2 (303-362) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	12	nm³

SASDQ26 – Retinoic acid-induced protein 2 (RAI2: 303-362) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-362: L319A, S320A) experimental SAS data

Retinoic acid-induced protein 2 (303-362: L319A, S320A) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-362: L319A, S320A) monomer, 7 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	2.3	nm
D_max	9.5	nm
Volume^Porod	10	nm³

SASDQ36 – Retinoic acid-induced protein 2 (RAI2: 303-362) M2 mutant L345A, S346A

Retinoic acid-induced protein 2 (303-362: L345A, S346A) experimental SAS data

Retinoic acid-induced protein 2 (303-362: L345A, S346A) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-362: L345A, S346A) monomer, 7 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	2.4	nm
D_max	10.0	nm
Volume^Porod	11	nm³

SASDQ46 – Retinoic acid-induced protein 2 (RAI2: 303-465)

Retinoic acid-induced protein 2 (303-465) experimental SAS data

Retinoic acid-induced protein 2 (303-465) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-465) monomer, 18 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	4.2	nm
D_max	14.5	nm
Volume^Porod	55	nm³

SASDQ56 – Retinoic acid-induced protein 2 (RAI2: 303-465) M1 mutant L319A, S320A

Retinoic acid-induced protein 2 (303-465: L319A, S320A) experimental SAS data

Retinoic acid-induced protein 2 (303-465: L319A, S320A) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-465: L319A, S320A) monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	4.2	nm
D_max	14.5	nm
Volume^Porod	54	nm³

SASDQ66 – Retinoic acid-induced protein 2 (RAI2: 303-465) M2 mutant L345A, S346A

Retinoic acid-induced protein 2 (303-465: L345A, S346A) experimental SAS data

Retinoic acid-induced protein 2 (303-465: L345A, S346A) Kratky plot

Sample:	Retinoic acid-induced protein 2 (303-465: L345A, S346A) monomer, 17 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	4.2	nm
D_max	14.5	nm
Volume^Porod	55	nm³

SASDQ76 – C-terminal-binding protein 1 (CtBP1) bound to Retinoic acid-induced protein 2 (RAI2: 303-362)

C-terminal-binding protein 1Retinoic acid-induced protein 2 (303-362) experimental SAS data

C-terminal-binding protein 1 Retinoic acid-induced protein 2 (303-362) Kratky plot

Sample:	C-terminal-binding protein 1 tetramer, 191 kDa Homo sapiens protein Retinoic acid-induced protein 2 (303-362) monomer, 7 kDa Homo sapiens protein
Buffer:	50 mM HEPES, 150 mM NaCl, 1 mM TCEP, 5% v/v glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 13

R_g^Guinier	7.4	nm
D_max	25.0	nm
Volume^Porod	889	nm³

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