Small Angle Scattering Biological Data Bank SASBDB

Browse by MACROMOLECULE type: protein

SASDQ29 – Thyroglobulin - reservoir sample storage with liquid jet delivery measured using XFEL

Sample:	Thyroglobulin monomer, 303 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

R_g^Guinier

7.0

SASDQ39 – SARS-CoV-2 spike protein ACE2 receptor binding domain (RBD) - reservoir sample storage with liquid jet delivery measured using XFEL

Spike glycoprotein (ACE2 receptor binding domain) experimental SAS data

Spike glycoprotein (ACE2 receptor binding domain) Kratky plot

Sample:	Spike glycoprotein (ACE2 receptor binding domain) monomer, 29 kDa Severe acute respiratory … protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

R_g^Guinier

3.0

SASDSE8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.27 mg/mL

Adhesion G-protein coupled receptor G4 experimental SAS data

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

The dimerized pentraxin-like domain of the adhesion G protein-coupled receptor 112 (ADGRG4) suggests function in sensing mechanical forces. J Biol Chem :105356 (2023)
Kieslich B, Weiße RH, Brendler J, Ricken A, Schöneberg T, Sträter N

R_g^Guinier	2.7	nm
D_max	7.1	nm
Volume^Porod	67	nm³

SASDSF8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 0.55 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	65	nm³

SASDSG8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.07 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	70	nm³

SASDSH8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 1.88 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.7	nm
Volume^Porod	74	nm³

SASDSJ8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 4.37 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.9	nm
Volume^Porod	76	nm³

SASDSK8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 6.38 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	3.0	nm
D_max	9.9	nm
Volume^Porod	77	nm³

SASDSL8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 7.51 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.9	nm
D_max	9.6	nm
Volume^Porod	77	nm³

SASDSM8 – Adhesion G protein-coupled receptor G4 PTX-like domain at 9.15 mg/mL

Sample:	Adhesion G-protein coupled receptor G4 dimer, 55 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 5% (w/v) glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 21

R_g^Guinier	2.9	nm
D_max	10.8	nm
Volume^Porod	78	nm³

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