Browse by MACROMOLECULE type: protein

SASDRA7 – HOTag6 tetramerization domain followed by a (Pro-Ala)2 linker fused to human monoubiquitin

HOTag6-(PA)2-Ubiquitin experimental SAS data
HOTag6-(PA)2-Ubiquitin Kratky plot
Sample: HOTag6-(PA)2-Ubiquitin tetramer, 52 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 15.0 nm
VolumePorod 74 nm3

SASDRB7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin

HOTag-(PA)4-Ubiquitin experimental SAS data
HOTag-(PA)4-Ubiquitin Kratky plot
Sample: HOTag-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 78 nm3

SASDRC7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin

HOTag-(PA)25-Ubiquitin experimental SAS data
HOTag-(PA)25-Ubiquitin Kratky plot
Sample: HOTag-(PA)25-Ubiquitin tetramer, 68 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 18.4 nm
VolumePorod 106 nm3

SASDRD7 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)-Ubiquitin experimental SAS data
HOTag6-(GS)-Ubiquitin Kratky plot
Sample: HOTag6-(GS)-Ubiquitin tetramer, 51 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 70 nm3

SASDRE7 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)4-Ubiquitin experimental SAS data
HOTag6-(GS)4-Ubiquitin Kratky plot
Sample: HOTag6-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 12.8 nm
VolumePorod 114 nm3

SASDRF7 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(GS)10-Ubiquitin experimental SAS data
HOTag6-(GS)10-Ubiquitin Kratky plot
Sample: HOTag6-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.2 nm
VolumePorod 86 nm3

SASDRG7 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)-Ubiquitin experimental SAS data
HOTag6-(PA)-Ubiquitin Kratky plot
Sample: HOTag6-(PA)-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.4 nm
VolumePorod 71 nm3

SASDRH7 – HOTag6 tetramerization domain followed by a (Pro-Ala)4 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)4-Ubiquitin experimental SAS data
HOTag6-(PA)4-Ubiquitin Kratky plot
Sample: HOTag6-(PA)4-Ubiquitin tetramer, 53 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.1 nm
Dmax 14.7 nm
VolumePorod 80 nm3

SASDRJ7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin with 200 mM NaCl

HOTag6-(PA)10-Ubiquitin experimental SAS data
HOTag6-(PA)10-Ubiquitin Kratky plot
Sample: HOTag6-(PA)10-Ubiquitin tetramer, 57 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 105 nm3

SASDRK7 – M1-linked tetraubiquitin, M1(1-72)-Ub4

Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 41 nm3