Browse by MACROMOLECULE type: protein

SASDSV8 – Tyrosine-protein kinase Src (c-Src WT)

Proto-oncogene tyrosine-protein kinase Src experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Proto-oncogene tyrosine-protein kinase Src monomer, 52 kDa Homo sapiens protein
Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 1% glycerol and 1 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2022 May 8
An allosteric switch between the activation loop and a c-terminal palindromic phospho-motif controls c-Src function Nature Communications 14(1) (2023)
Cuesta-Hernández H, Contreras J, Soriano-Maldonado P, Sánchez-Wandelmer J, Yeung W, Martín-Hurtado A, Muñoz I, Kannan N, Llimargas M, Muñoz J, Plaza-Menacho I
RgGuinier 2.6 nm
Dmax 8.9 nm
VolumePorod 67 nm3

SASDSZ8 – The DNA binding domain (DBD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 23 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 1 mM TCEP,, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 25
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 2.7 nm
Dmax 10.2 nm
VolumePorod 22 nm3

SASDS29 – The DNA binding and transactivation domains (DBD-TAD) of Hepatocyte nuclear factor 1-alpha (HNF-1A)

Hepatocyte nuclear factor 1-alpha experimental SAS data
Hepatocyte nuclear factor 1-alpha Kratky plot
Sample: Hepatocyte nuclear factor 1-alpha monomer, 62 kDa Homo sapiens protein
Buffer: 4 mM Tris, 100 mM NaCl, 1 mM TCEP,, pH: 8.5
Experiment: SAXS data collected at CoSAXS, MAX IV on 2022 Apr 2
Structural properties of the HNF-1A transactivation domain Frontiers in Molecular Biosciences 10 (2023)
Kind L, Driver M, Raasakka A, Onck P, Njølstad P, Arnesen T, Kursula P
RgGuinier 4.9 nm
Dmax 17.0 nm
VolumePorod 109 nm3

SASDR37 – HOTag6 tetramerization domain followed by a Gly-Ser linker fused to human monoubiquitin

HOTag-GS-Ubiquitin experimental SAS data
HOTag-GS-Ubiquitin Kratky plot
Sample: HOTag-GS-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 14.0 nm
VolumePorod 74 nm3

SASDR47 – HOTag6 tetramerization domain followed by a (Gly-Ser)2 linker fused to human monoubiquitin

HOTag6-(GS)2-Ubiquitin experimental SAS data
HOTag6-(GS)2-Ubiquitin Kratky plot
Sample: HOTag6-(GS)2-Ubiquitin tetramer, 52 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 13.7 nm
VolumePorod 69 nm3

SASDR57 – HOTag6 tetramerization domain followed by a (Gly-Ser)4 linker fused to human monoubiquitin

HOTag-(GS)4-Ubiquitin experimental SAS data
HOTag-(GS)4-Ubiquitin Kratky plot
Sample: HOTag-(GS)4-Ubiquitin tetramer, 53 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.8 nm
Dmax 14.3 nm
VolumePorod 76 nm3

SASDR67 – HOTag6 tetramerization domain followed by a (Gly-Ser)10 linker fused to human monoubiquitin

HOTag-(GS)10-Ubiquitin experimental SAS data
HOTag-(GS)10-Ubiquitin Kratky plot
Sample: HOTag-(GS)10-Ubiquitin tetramer, 56 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.0 nm
Dmax 14.8 nm
VolumePorod 84 nm3

SASDR77 – HOTag6 tetramerization domain followed by a (Gly-Ser)25 linker fused to human monoubiquitin

HOTag6-(GS)25-Ubiquitin experimental SAS data
HOTag6-(GS)25-Ubiquitin Kratky plot
Sample: HOTag6-(GS)25-Ubiquitin tetramer, 65 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 21
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.7 nm
Dmax 19.3 nm
VolumePorod 117 nm3

SASDR87 – HOTag6 tetramerization domain followed by a (Gly-Ser)50 linker fused to human monoubiquitin

HOTag-(GS)50-Ubiquitin experimental SAS data
HOTag-(GS)50-Ubiquitin Kratky plot
Sample: HOTag-(GS)50-Ubiquitin tetramer, 80 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.6 nm
Dmax 22.7 nm
VolumePorod 193 nm3

SASDR97 – HOTag6 tetramerization domain followed by a Pro-Ala linker fused to human monoubiquitin

HOTag-PA-Ubiquitin experimental SAS data
HOTag-PA-Ubiquitin Kratky plot
Sample: HOTag-PA-Ubiquitin tetramer, 51 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 16
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.7 nm
Dmax 12.9 nm
VolumePorod 73 nm3