Browse by ORGANISM: Homo sapiens (Human)

SASDN53 – Ternary Human Pex5p(full-length)-Pex14p(N)-PTS1 Protein Complex (1:7:1 stoichiometry)

Peroxisomal targeting signal 1 receptorPeroxisomal membrane protein PEX14PTS1-BP experimental SAS data
SASREF model
Sample: Peroxisomal targeting signal 1 receptor monomer, 71 kDa Homo sapiens protein
Peroxisomal membrane protein PEX14 monomer, 7 kDa Homo sapiens protein
PTS1-BP monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 18
Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. J Biol Chem 284(37):25334-42 (2009)
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 267 nm3

SASDNZ2 – Tetrameric state of soluble endoglin receptor

Endoglin experimental SAS data
DAMMIF model
Sample: Endoglin tetramer, 243 kDa Homo sapiens protein
Buffer: 10 mM Tris–HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Oct 17
Structural and functional characterization of soluble endoglin receptor Biochemical and Biophysical Research Communications 383(4):386-391 (2009)
Le B, Franke D, Svergun D, Han T, Hwang H, Kim K
RgGuinier 7.6 nm
Dmax 26.0 nm
VolumePorod 434 nm3

SASDN23 – Homodimeric state of soluble endoglin receptor

Endoglin experimental SAS data
DAMMIF model
Sample: Endoglin dimer, 121 kDa Homo sapiens protein
Buffer: 10 mM Tris–HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Oct 17
Structural and functional characterization of soluble endoglin receptor Biochemical and Biophysical Research Communications 383(4):386-391 (2009)
Le B, Franke D, Svergun D, Han T, Hwang H, Kim K
RgGuinier 4.7 nm
Dmax 17.0 nm
VolumePorod 173 nm3

SASDMC7 – Nuclear fragile X mental retardation-interacting protein 2 (FRMP)

Nuclear fragile X mental retardation-interacting protein 2 experimental SAS data
DAMMIN model
Sample: Nuclear fragile X mental retardation-interacting protein 2 monomer, 59 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl pH 8.0, 5 mM TCEP, pH:
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 11
A study of the ultrastructure of fragile-X-related proteins. Biochem J 419(2):347-57 (2009)
Sjekloća L, Konarev PV, Eccleston J, Taylor IA, Svergun DI, Pastore A
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 92 nm3

SASDLX5 – Wild-type human mitochondrial deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial experimental SAS data
BUNCH model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial trimer, 80 kDa Homo sapiens protein
Buffer: 20 mM citrate buffer, 1 mM DTT, 0.1 mM PMSF and 1 mM MgCl2, pH: 5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Feb 1
Molecular shape and prominent role of β-strand swapping in organization of dUTPase oligomers FEBS Letters 583(5):865-871 (2009)
Takács E, Barabás O, Petoukhov M, Svergun D, Vértessy B
RgGuinier 2.6 nm
Dmax 7.0 nm
VolumePorod 101 nm3

SASDNC2 – NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12

Macrophage metalloelastase experimental SAS data
NNGH-inhibited, cadmium(II)-substituted Phe171Asp/Glu219Ala double mutant of FL-MMP- 12 Rg histogram
Sample: Macrophage metalloelastase monomer, 54 kDa Homo sapiens protein
Buffer: 20 mM Tris10 mM CaCl2, 0.3 M NaCl, 0.2 M AHA, pH: 7.2
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Oct 2
Evidence of Reciprocal Reorientation of the Catalytic and Hemopexin-Like Domains of Full-Length MMP-12 Journal of the American Chemical Society 130(22):7011-7021 (2008)
Bertini I, Calderone V, Fragai M, Jaiswal R, Luchinat C, Melikian M, Mylonas E, Svergun D
RgGuinier 3.2 nm
Dmax 11.0 nm
VolumePorod 58 nm3

SASDLZ5 – Hepatocyte growth factor/Hepatocyte growth factor receptor (NK1-Met567) complex

Hepatocyte growth factorHepatocyte growth factor receptor experimental SAS data
MONSA model
Sample: Hepatocyte growth factor dimer, 39 kDa Homo sapiens protein
Hepatocyte growth factor receptor dimer, 122 kDa Homo sapiens protein
Buffer: Phosphate-buffered saline containing EDTA, surfactant P20 and 5 M NaCl, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jun 26
Engineering the NK1 Fragment of Hepatocyte Growth Factor/Scatter Factor as a MET Receptor Antagonist Journal of Molecular Biology 377(3):616-622 (2008)
Youles M, Holmes O, Petoukhov M, Nessen M, Stivala S, Svergun D, Gherardi E
RgGuinier 5.3 nm
Dmax 20.0 nm
VolumePorod 290 nm3

SASDNW2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268)

Fab fragment in complex with small molecule hapten, crystal form-1 experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 71 nm3

SASDNX2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) bound to TSC ligand

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data
DAMMIN model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 27
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 4.1 nm
Dmax 16.0 nm
VolumePorod 139 nm3

SASDNY2 – Human Anti-TSC Recombinant Antibody (Fab MOR03268) with TSC ligand excess

Fab fragment in complex with small molecule hapten, crystal form-1(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Fab fragment in complex with small molecule hapten, crystal form-1 monomer, 45 kDa Homo sapiens protein
(1S)-1-AMINO-2-(1H-INDOL-3-YL)ETHANOL monomer, 0 kDa
Buffer: 20 mM Tris–HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Jul 25
Fab MOR03268 Triggers Absorption Shift of a Diagnostic Dye via Packaging in a Solvent-shielded Fab Dimer Interface Journal of Molecular Biology 377(1):206-219 (2008)
Hillig R, Urlinger S, Fanghänel J, Brocks B, Haenel C, Stark Y, Sülzle D, Svergun D, Baesler S, Malawski G, Moosmayer D, Menrad A, Schirner M, Licha K
RgGuinier 3.6 nm
Dmax 15.0 nm
VolumePorod 107 nm3