Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDAN4 – Calmodulin:peptide complex

CalmodulinC-terminal region of human myelin basic protein experimental SAS data

Sample:	Calmodulin monomer, 17 kDa Homo sapiens protein C-terminal region of human myelin basic protein monomer, 2 kDa Homo sapiens protein
Buffer:	25 mM Tris75 200 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 28

Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure. BMC Struct Biol 8:10 (2008)
Majava V, Petoukhov MV, Hayashi N, Pirilä P, Svergun DI, Kursula P

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	36	nm³

SASDAK5 – Myomesin-1 My12-My13

Sample:	Myomesin-1 dimer, 46 kDa Homo sapiens protein
Buffer:	25 mM Tris/HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 7

Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin. EMBO J 27(1):253-64 (2008)
Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M

R_g^Guinier	4.0	nm
D_max	14.5	nm
Volume^Porod	56	nm³

SASDNN2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb2) at concentration c = 21 mg/ml

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG experimental SAS data

Human hemoglobin conjugated with six-seven copies of 5-kDa PEG Kratky plot

Sample:	Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

Solution Structure of Poly(ethylene) Glycol-Conjugated Hemoglobin Revealed by Small-Angle X-Ray Scattering: Implications for a New Oxygen Therapeutic Biophysical Journal 94(1):173-181 (2008)
Svergun D, Ekström F, Vandegriff K, Malavalli A, Baker D, Nilsson C, Winslow R

R_g^Guinier

3.0

SASDNP2 – Experimental SAXS data for hemoglobin conjucted with six-seven copies of PEG dimer (Hb5)

Sample:	Human hemoglobin conjugated with six-seven copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	3.3	nm
D_max	13.0	nm

SASDNQ2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2) at concentration c = 25 mg/ml

Human hemoglobin conjugated with two copies of 5-kDa PEG experimental SAS data

Human hemoglobin conjugated with two copies of 5-kDa PEG Kratky plot

Sample:	Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier

2.4

SASDNR2 – Experimental SAXS data for hemoglobin conjucted with two copies of PEG dimer (Hb2)

Sample:	Human hemoglobin conjugated with two copies of 5-kDa PEG dimer, 62 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	2.8	nm
D_max	13.0	nm

SASDNS2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 5 mg/ml

Hemoglobin subunit alphaHemoglobin subunit beta experimental SAS data

Sample:	Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier	2.4	nm
D_max	13.0	nm

SASDNT2 – Experimental SAXS data for native hemoglobin (Hb) at concentration c = 31.25 mg/ml

Hemoglobin subunit alpha Hemoglobin subunit beta Kratky plot

Sample:	Hemoglobin subunit alpha monomer, 15 kDa Homo sapiens protein Hemoglobin subunit beta monomer, 16 kDa Homo sapiens protein
Buffer:	Ringer's lactate solution, pH: 6.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 19

R_g^Guinier

2.2

SASDL27 – N-Oct-3 POU transcription factor domain in complex with rat CRH DNA

POU domain, class 3, transcription factor 2Rat CRH DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Rat CRH DNA monomer, 15 kDa Rattus norvegicus DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Oct 9

Fine-tuning of intrinsic N-Oct-3 POU domain allostery by regulatory DNA targets Nucleic Acids Research 35(13):4420-4432 (2007)
Alazard R, Mourey L, Ebel C, Konarev P, Petoukhov M, Svergun D, Erard M

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	41	nm³

SASDL37 – N-Oct-3 POU transcription factor domain in complex with human DR-alpha DNA

POU domain, class 3, transcription factor 2Human DR-alpha DNA experimental SAS data

Sample:	POU domain, class 3, transcription factor 2 monomer, 19 kDa Homo sapiens protein Human DR-alpha DNA monomer, 15 kDa Homo sapiens DNA
Buffer:	50 mM Tris, 0.4 M NaCl, 2% glycerol, 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2004 May 26

R_g^Guinier	2.9	nm
D_max	11.0	nm
Volume^Porod	44	nm³

First << Prev Page 154 of 156 Next >> Last