Browse by ORGANISM: Homo sapiens (Human)

SASDJW7 – pri-miR16-1 primary microRNA in complex with DGCR8-core protein

primary microRNA pri-miR16-1 complexed with DGCR8-core proteinMicroprocessor complex subunit DGCR8 experimental SAS data
DAMMIF model
Sample: Primary microRNA pri-miR16-1 complexed with DGCR8-core protein monomer, 36 kDa Homo sapiens RNA
Microprocessor complex subunit DGCR8 monomer, 26 kDa Homo sapiens protein
Buffer: 50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, 50% sucrose, DGCR8-core, pH: 7.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12
Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S
RgGuinier 5.0 nm
Dmax 17.2 nm
VolumePorod 125 nm3

SASDJ47 – Polyglutamine protein ataxin-3 (Q13)

Ataxin-3 (polyglutamine protein ataxin-3 (Q13)) experimental SAS data
Polyglutamine protein ataxin-3 (Q13) Rg histogram
Sample: Ataxin-3 (polyglutamine protein ataxin-3 (Q13)) monomer, 41 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate buffer, 2 mM TCEP, 5% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 24
Capturing the Conformational Ensemble of the Mixed Folded Polyglutamine Protein Ataxin-3. Structure (2020)
Sicorello A, Różycki B, Konarev PV, Svergun DI, Pastore A
RgGuinier 4.0 nm
Dmax 14.1 nm
VolumePorod 79 nm3

SASDFS7 – Talin-1 head amino acids 1-405(Δ139-168)

Talin-1 (Δ139-168), human experimental SAS data
Talin-1 head amino acids 1-405(Δ139-168) Rg histogram
Sample: Talin-1 (Δ139-168), human monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24
The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci 133(19) (2020)
Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B
RgGuinier 3.3 nm
Dmax 13.3 nm
VolumePorod 77 nm3

SASDFT7 – Talin-1 head amino acids 1-405

Talin-1, human experimental SAS data
Talin-1 head amino acids 1-405 Rg histogram
Sample: Talin-1, human monomer, 51 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24
The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci 133(19) (2020)
Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B
RgGuinier 3.4 nm
Dmax 11.5 nm
VolumePorod 94 nm3

SASDFU7 – Talin-1 head amino acids 1-405(Δ134-170/GAG insert)

Talin-1 (Δ134-170/GAG insert), human experimental SAS data
Talin-1 head amino acids 1-405(Δ134-170/GAG insert) Rg histogram
Sample: Talin-1 (Δ134-170/GAG insert), human monomer, 47 kDa Homo sapiens protein
Buffer: 50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24
The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering. J Cell Sci 133(19) (2020)
Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B
RgGuinier 3.0 nm
Dmax 10.2 nm
VolumePorod 71 nm3

SASDNU3 – Fibrillin PF2

Fibrillin-1 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2006 Feb 11
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 84 nm3

SASDNV3 – Synthetic Human Elastin without domain 26A

Elastin experimental SAS data
Elastin Kratky plot
Sample: Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2011 Mar 15
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 6.6 nm
Dmax 22.5 nm

SASDNW3 – Fibrillin-1 fragment PF2 crosslinked to tropoelastin by transglutaminase 2

Fibrillin-1Elastin experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 21
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 7.1 nm
Dmax 23.5 nm

SASDJF3 – Aldo-keto reductase family 1 member B1 (AKR1B1) in the presence of 100 mM 5-methyl uridine

Aldo-keto reductase family 1 member B1 experimental SAS data
Aldo-keto reductase family 1 member B1 Kratky plot
Sample: Aldo-keto reductase family 1 member B1 monomer, 36 kDa Homo sapiens protein
Buffer: 20 mM Tris, 250 mM NaCl, 100 mM 5-methyl uridine, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Jun 14
Improving data quality and expanding BioSAXS experiments to low-molecular-weight and low-concentration protein samples. Acta Crystallogr D Struct Biol 76(Pt 10):971-981 (2020)
Castellví A, Pascual-Izarra C, Crosas E, Malfois M, Juanhuix J
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 52 nm3

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag

his-trx-myotilin experimental SAS data
Full-length myotilin with N-terminally fused thioredoxin tag Rg histogram
Sample: His-trx-myotilin monomer, 70 kDa Homo sapiens protein
Buffer: 20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11
Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
RgGuinier 5.1 nm
Dmax 16.7 nm
VolumePorod 131 nm3