Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452)

Myotilin Ig1Ig2 (220-452) experimental SAS data

Sample:	Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2010 Nov 16

Molecular basis of F-actin regulation and sarcomere assembly via myotilin PLOS Biology 19(4):e3001148 (2021)
Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L

R_g^Guinier	3.7	nm
D_max	15.1	nm
Volume^Porod	52	nm³

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444)

Myotilin Ig1Ig2 (250-444) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	2.8	nm
D_max	10.1	nm
Volume^Porod	29	nm³

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498)

Myotilin Ig1Ig2 (250-498) experimental SAS data

Sample:	Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein
Buffer:	20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at BM29, ESRF on 2019 Jun 27

R_g^Guinier	3.2	nm
D_max	14.0	nm
Volume^Porod	37	nm³

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data

Myotilin (222-452) experimental SAS data

Sample:	Myotilin (222-452) monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6

R_g^Guinier	3.3	nm
D_max	11.5	nm
Volume^Porod	36	nm³

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data

Myotilin (222-452) R405K experimental SAS data

Sample:	Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12

R_g^Guinier	3.3	nm
D_max	10.5	nm
Volume^Porod	36	nm³

SASDJU4 – X-ray repair cross-complementing proteins 5 and 6

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5 experimental SAS data

Sample:	X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein
Buffer:	50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12

Visualizing functional dynamicity in the DNA-dependent protein kinase holoenzyme DNA-PK complex by integrating SAXS with cryo-EM. Prog Biophys Mol Biol (2020)
Hammel M, Rosenberg DJ, Bierma J, Hura GL, Lees-Miller SP, Tainer JA

R_g^Guinier	4.2	nm
D_max	14.9	nm
Volume^Porod	253	nm³

SASDJV4 – X-ray repair cross-complementing proteins 5ΔCTR and 6

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5 ΔCTR experimental SAS data

Sample:	X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein X-ray repair cross-complementing protein 5 ΔCTR monomer, 64 kDa Homo sapiens protein
Buffer:	50 mM Hepes, 50 mM KCl, 5 mM MgCl2, 5% glycerol and 0.2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Mar 12

R_g^Guinier	3.8	nm
D_max	12.5	nm
Volume^Porod	240	nm³

SASDJW4 – X-ray repair cross-complementing proteins 5 and 6-DNA

X-ray repair cross-complementing protein 6X-ray repair cross-complementing protein 5Y-DNA experimental SAS data

Sample:	X-ray repair cross-complementing protein 6 monomer, 70 kDa Homo sapiens protein X-ray repair cross-complementing protein 5 monomer, 83 kDa Homo sapiens protein Y-DNA monomer, 18 kDa DNA
Buffer:	50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Jan 8

R_g^Guinier	4.1	nm
D_max	14.8	nm
Volume^Porod	280	nm³

SASDJX4 – DNA-dependent protein kinase catalytic subunit (DNA-PKcs)

DNA-dependent protein kinase catalytic subunit experimental SAS data

Sample:	DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 100 mM NaCl, 5% glycerol, 0.01% sodium azide, pH: 7.5
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 30

R_g^Guinier	5.6	nm
D_max	15.6	nm
Volume^Porod	1040	nm³

SASDJY4 – Autophosphorylated DNA-dependent protein kinase (DNA-PKcs)

Sample:	DNA-dependent protein kinase catalytic subunit monomer, 468 kDa Homo sapiens protein
Buffer:	50 mM Tris-HCl, 100 mM KCl, 5% (v/v) glycerol, 0.2 mM EDTA containing 0.1 mM benzamidine, 0.2 mM PMSF and 0.2 µg/ml pepstatin, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2010 Jan 8

R_g^Guinier	5.5	nm
D_max	16.1	nm
Volume^Porod	918	nm³

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