Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Homo sapiens (Human)

SASDGV9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783

Nucleolar RNA helicase 2 fragment 186-783 experimental SAS data

Sample:	Nucleolar RNA helicase 2 fragment 186-783 dimer, 138 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M

R_g^Guinier	4.8	nm
D_max	17.6	nm
Volume^Porod	297	nm³

SASDGW9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-710

Nucleolar RNA helicase 2 fragment 186-710 experimental SAS data

Nucleolar RNA helicase 2 (DDX21) fragment 186-710 Rg histogram

Sample:	Nucleolar RNA helicase 2 fragment 186-710 dimer, 121 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

R_g^Guinier	4.6	nm
D_max	17.7	nm
Volume^Porod	238	nm³

SASDGX9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-620

Nucleolar RNA helicase 2 fragment 186-620 experimental SAS data

Sample:	Nucleolar RNA helicase 2 fragment 186-620 dimer, 98 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Mar 4

R_g^Guinier	4.4	nm
D_max	16.9	nm
Volume^Porod	150	nm³

SASDGY9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant

Nucleolar RNA helicase 2 experimental SAS data

Sample:	Nucleolar RNA helicase 2 monomer, 69 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2018 Nov 3

R_g^Guinier	4.1	nm
D_max	17.7	nm
Volume^Porod	111	nm³

SASDHL9 – Full-length HERV-K Rec response element

Full-length HERV-K Rec response element experimental SAS data

Full-length HERV-K Rec response element Kratky plot

Sample:	Full-length HERV-K Rec response element monomer, 141 kDa Homo sapiens RNA
Buffer:	10mM Tris-HCl, 100mM KCl, and 0.5mM MgCl₂, pH: 8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Feb 10

Structural Mimicry Drives HIV-1 Rev-Mediated HERV-K Expression Journal of Molecular Biology 432(24):166711 (2020)
O'Carroll I, Fan L, Kroupa T, McShane E, Theodore C, Yates E, Kondrup B, Ding J, Martin T, Rein A, Wang Y

R_g^Guinier	7.5	nm
D_max	28.2	nm
Volume^Porod	454	nm³

SASDHM9 – Truncated HERV-K Rec response element (RcRE) RNA

Truncated HERV-K Rec response element experimental SAS data

Sample:	Truncated HERV-K Rec response element monomer, 102 kDa Homo sapiens RNA
Buffer:	10mM Tris-HCl, 100mM KCl, and 0.5mM MgCl₂, pH: 8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Jul 17

R_g^Guinier	6.1	nm
D_max	21.8	nm
Volume^Porod	322	nm³

SASDHN9 – TrΔSLIII HERV-K Rec response element (RcRE) RNA

TrΔSLIII HERV-K Rec response element experimental SAS data

Sample:	TrΔSLIII HERV-K Rec response element monomer, 88 kDa Homo sapiens RNA
Buffer:	10mM Tris-HCl, 100mM KCl, and 0.5mM MgCl₂, pH: 8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Dec 7

R_g^Guinier	5.3	nm
D_max	19.2	nm
Volume^Porod	226	nm³

SASDHP9 – TrΔSLIIab HERV-K Rec response element (RcRE) RNA

TrΔSLIIab HERV-K Rec response element experimental SAS data

Sample:	TrΔSLIIab HERV-K Rec response element monomer, 86 kDa Homo sapiens RNA
Buffer:	10mM Tris-HCl, 100mM KCl, and 0.5mM MgCl₂, pH: 8
Experiment:	SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 21

R_g^Guinier	5.0	nm
D_max	18.4	nm
Volume^Porod	201	nm³

SASDJQ7 – Human Cathepsin X

Sample:	Cathepsin Z dimer, 54 kDa Homo sapiens protein
Buffer:	20 mM sodium acetate, 1 mM EDTA, pH: 5.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Dec 6

Human cathepsin X/Z is a biologically active homodimer. Biochim Biophys Acta Proteins Proteom :140567 (2020)
Dolenc I, Štefe I, Turk D, Taler-Verčič A, Turk B, Turk V, Stoka V

R_g^Guinier	2.6	nm
D_max	6.5	nm
Volume^Porod	89	nm³

SASDJV7 – pri-miR16-1 primary microRNA

primary microRNA pri-miR16-1 experimental SAS data

Sample:	Primary microRNA pri-miR16-1 monomer, 36 kDa Homo sapiens RNA
Buffer:	50 mM KCl, 50 mM HEPES, 5 mM DTT, 1% glycerol, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Apr 12

Elucidating the Role of Microprocessor Protein DGCR8 in Bending RNA Structures Biophysical Journal (2020)
Pabit S, Chen Y, Usher E, Cook E, Pollack L, Showalter S

R_g^Guinier	4.4	nm
D_max	17.2	nm
Volume^Porod	74	nm³

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