Browse by ORGANISM: Mus musculus (Mouse)

SASDHC5 – Nuclear factor kB p50/RelA heterodimer

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350 experimental SAS data
BILBOMD model
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.6 nm
Dmax 15.3 nm
VolumePorod 183 nm3

SASDHD5 – Transactivation domain of RelA

Transcription factor p65 340-549 experimental SAS data
MULTIFOXS model
Sample: Transcription factor p65 340-549 monomer, 23 kDa Mus musculus protein
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 2.7 nm
Dmax 8.2 nm
VolumePorod 59 nm3

SASDHE5 – Rel Homology Domain of p50/RelA bound to IFN kB site DNA

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321IFN kB DNA experimental SAS data
NF-kappa-B p105 subunit 39-350 Transcription factor p65 19-321 IFN kB DNA Kratky plot
Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
IFN kB DNA monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.3 nm
Dmax 13.0 nm
VolumePorod 237 nm3

SASDHF5 – Rel Homology Domain of p50/RelA bound to Urokinase kB site DNA

NF-kappa-B p105 subunit 39-350Transcription factor p65 19-321Urokinase kB experimental SAS data
NF-kappa-B p105 subunit 39-350 Transcription factor p65 19-321 Urokinase kB Kratky plot
Sample: NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Transcription factor p65 19-321 monomer, 35 kDa Mus musculus protein
Urokinase kB monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Jun 11
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.1 nm
Dmax 13.0 nm
VolumePorod 244 nm3

SASDHG5 – Nuclear factor kB p50/RelA bound to IFN kB site DNA

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350IFN kB DNA experimental SAS data
Transcription factor p65 19-549 NF-kappa-B p105 subunit 39-350 IFN kB DNA Kratky plot
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
IFN kB DNA monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 5.1 nm
Dmax 18.9 nm
VolumePorod 314 nm3

SASDHH5 – Nuclear factor kB p50/RelA bound to Urokinase kB site DNA

Transcription factor p65 19-549NF-kappa-B p105 subunit 39-350Urokinase kB experimental SAS data
Transcription factor p65 19-549 NF-kappa-B p105 subunit 39-350 Urokinase kB Kratky plot
Sample: Transcription factor p65 19-549 monomer, 58 kDa Mus musculus protein
NF-kappa-B p105 subunit 39-350 monomer, 36 kDa Mus musculus protein
Urokinase kB monomer, 8 kDa DNA
Buffer: 25 mM Tris.Cl, 150 mM NaCl, 1 mM DTT, 0.5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 Dec 4
An intrinsically disordered transcription activation domain increases the DNA binding affinity and reduces the specificity of NFκB p50/RelA. J Biol Chem :102349 (2022)
Baughman HER, Narang D, Chen W, Villagrán Suárez AC, Lee J, Bachochin MJ, Gunther TR, Wolynes PG, Komives EA
RgGuinier 4.7 nm
Dmax 17.5 nm
VolumePorod 201 nm3

SASDNW5 – apo Roquin-1 (coreROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 experimental SAS data
Roquin-1 Kratky plot
Sample: Roquin-1 monomer, 18 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 1.9 nm
Dmax 7.0 nm
VolumePorod 35 nm3

SASDNX5 – apo Roquin-1 (extROQ) at 50 µM, including concentration series data at 100, 200, 300 µM

Roquin-1 extROQ experimental SAS data
Roquin-1 extROQ Kratky plot
Sample: Roquin-1 extROQ monomer, 35 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 2.8 nm
Dmax 14.0 nm
VolumePorod 53 nm3

SASDNY5 – apo Roquin-1 (Nterm ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm experimental SAS data
Roquin-1 Nterm Kratky plot
Sample: Roquin-1 Nterm monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 3.4 nm
Dmax 15.0 nm
VolumePorod 88 nm3

SASDNZ5 – apo Roquin-1 (Nterm A-site mut ROQ) at 50 µM, including concentration series data at 100, 300 µM

Roquin-1 Nterm Amut (K220A, K239A, R260A) experimental SAS data
Roquin-1 Nterm Amut (K220A, K239A, R260A) Kratky plot
Sample: Roquin-1 Nterm Amut (K220A, K239A, R260A) monomer, 51 kDa Mus musculus protein
Buffer: 150 mM NaCl, 20 mM Tris, 2 mM TCEP, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 1
NMR-derived secondary structure of the full-length Ox40 mRNA 3'UTR and its multivalent binding to the immunoregulatory RBP Roquin. Nucleic Acids Res (2022)
Tants JN, Becker LM, McNicoll F, Müller-McNicoll M, Schlundt A
RgGuinier 3.3 nm
Dmax 14.0 nm
VolumePorod 80 nm3