Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: Mus musculus (Mouse)

SASDF96 – Olfactomedin-1 BMY isoform

Sample:	Noelin tetramer, 72 kDa Mus musculus protein
Buffer:	150 mM NaCl, 20 mM HEPES, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2016 Feb 5

Design and structural characterisation of olfactomedin-1 variants as tools for functional studies. BMC Mol Cell Biol 20(1):50 (2019)
Pronker MF, van den Hoek H, Janssen BJC

R_g^Guinier	5.4	nm
D_max	16.3	nm
Volume^Porod	160	nm³

SASDG64 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes. Structure (2019)
Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	194	nm³

SASDG74 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF and GASBOR models)

Sample:	Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.3	nm
D_max	25.7	nm
Volume^Porod	168	nm³

SASDG84 – Autoinhibited dimer of truncated 6xHis Cytohesin-2 (ARNO, amino acids 2-400) with Inositol 1,3,4,5-tetrakis phosphate (DAMMIF, GASBOR and antiparallel CORAL models)

Sample:	Cytohesin-2 dimer, 95 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.3	nm
D_max	27.0	nm
Volume^Porod	180	nm³

SASDG94 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Sample:	Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	194	nm³

SASDGA4 – Autoinhibited dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-399) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Sample:	Cytohesin-3 dimer, 93 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.5	nm
D_max	26.0	nm
Volume^Porod	194	nm³

SASDGB4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (antiparallel CORAL and MultiFoXS models)

Sample:	Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.1	nm
D_max	25.7	nm
Volume^Porod	168	nm³

SASDGC4 – Active dimer of truncated 6xHis Cytohesin-3 (Grp1, amino acids 14-390) with Inositol 1,3,4,5-tetrakis phosphate (parallel CORAL and MultiFoXS models)

Sample:	Cytohesin-3 dimer, 90 kDa Mus musculus protein
Buffer:	20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15

R_g^Guinier	5.1	nm
D_max	25.7	nm
Volume^Porod	168	nm³

SASDF27 – Brain and Muscle ARNT-Like 1 from Mus musculus (D530-L625), monomer, trans-conformation locking mutation P624A

Aryl hydrocarbon receptor nuclear translocator-like protein 1 experimental SAS data

Sample:	Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein
Buffer:	25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 27

Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein. J Biol Chem (2019)
Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E

R_g^Guinier	2.8	nm
D_max	11.0	nm

SASDF37 – KIX domain (kinase-inducible domain interacting domain) of CREB-binding protein (Mus musculus), mutation L664C

Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C experimental SAS data

Sample:	Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein
Buffer:	25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 May 27

R_g^Guinier	1.9	nm
D_max	8.1	nm

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