Browse by ORGANISM: Mus musculus (Mouse)

SASDCB9 – Nanolipoprotein Particle (NLP) assembled with tail-deuterated DMPC - 100% D2O SANS

Apolipoprotein A-I1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-d54-sn-glycero-3-phosphocholine, 99 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2016 Apr 11
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.3 nm
Dmax 10.7 nm

SASDCC9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 42% D2O SANS

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in 42% D2O, pH: 7.4
Experiment: SANS data collected at NGB 30m SANS, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Dec 11
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 2.9 nm
Dmax 7.3 nm

SASDCD9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - SAXS

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.7 nm
Dmax 12.7 nm

SASDCE9 – Nanolipoprotein Particle (NLP) with Telodendrimer - SAXS

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.4 nm
Dmax 12.7 nm

SASDCF9 – Nanolipoprotein Particle (NLP) from Cell-Free Expression - 100% D2O SANS

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.5 nm
Dmax 12.5 nm

SASDCG9 – Nanolipoprotein Particle (NLP) with Telodendrimer - 100% D2O SANS

1,2-dimyristoyl-sn-glycero-3-phosphocholineApolipoprotein A-I experimental SAS data
1,2-dimyristoyl-sn-glycero-3-phosphocholine Apolipoprotein A-I Kratky plot
Sample: 1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Apolipoprotein A-I dimer, 52 kDa Mus musculus protein
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.3 nm
Dmax 10.0 nm

SASDCK7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399)

Cytohesin-3 experimental SAS data
DAMMIF model
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 13
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 2.8 nm
Dmax 11.2 nm
VolumePorod 61 nm3

SASDCL7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390)

Cytohesin-3 experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-390) Rg histogram
Sample: Cytohesin-3 monomer, 40 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.001 mM inositol 1,3,4,5-tetrakisphosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Mar 21
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 2.8 nm
Dmax 9.3 nm
VolumePorod 64 nm3

SASDCM7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein experimental SAS data
DAMMIF model
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L fusion protein monomer, 61 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.3 nm
Dmax 11.9 nm
VolumePorod 93 nm3

SASDCN7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein experimental SAS data
Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein Kratky plot
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein monomer, 62 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.4 nm
Dmax 13.6 nm
VolumePorod 101 nm3