Browse by ORGANISM: Mus musculus (Mouse)

SASDCP7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L SUMO fusion protein

Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein experimental SAS data
DAMMIF model
Sample: Grp1 63-399 E161A 6GS Arf6 Q67L SUMO fusion protein monomer, 72 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.8 nm
Dmax 14.5 nm
VolumePorod 116 nm3

SASDCQ7 – Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein

Grp1 63-399 E161A Arf6 Q67L fusion protein experimental SAS data
Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A Arf6 Q67L fusion protein Rg histogram
Sample: Grp1 63-399 E161A Arf6 Q67L fusion protein monomer, 60 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate, pH: 8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2013 Nov 15
Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors. Structure 26(1):106-117.e6 (2018)
Malaby AW, Das S, Chakravarthy S, Irving TC, Bilsel O, Lambright DG
RgGuinier 3.1 nm
Dmax 11.6 nm
VolumePorod 86 nm3

SASDCW5 – Dimeric Sortilin at pH 7.4

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
DAMMIF model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 275 nm3

SASDCX5 – Monomeric Sortilin at pH 5.5

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
DAMMIF model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.4 nm
Dmax 10.0 nm
VolumePorod 217 nm3

SASDCY5 – Dimeric Sortilin at pH 5.5

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
CORAL model
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM MES pH 5.5, 150 mM NaCl, pH: 5.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.9 nm
Dmax 11.0 nm
VolumePorod 336 nm3

SASDCZ5 – Monomeric Sortilin at pH 7.4

Sortilin 1 A464E alias Neurotensin-receptor 3 A464E experimental SAS data
CORAL model
Sample: Sortilin 1 A464E alias Neurotensin-receptor 3 A464E monomer, 76 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 10.5 nm
VolumePorod 217 nm3

SASDCE7 – Monomeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.3 nm
Dmax 11.7 nm
VolumePorod 192 nm3

SASDCF7 – Dimeric Sortilin at pH 7.4 in the presence of neurotensin

Sortilin, also: Neurotensin-receptor 3 experimental SAS data
Sortilin, also: Neurotensin-receptor 3 Kratky plot
Sample: Sortilin, also: Neurotensin-receptor 3 dimer, 153 kDa Mus musculus protein
Buffer: 25 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Apr 17
Low pH-induced conformational change and dimerization of sortilin triggers endocytosed ligand release. Nat Commun 8(1):1708 (2017)
Leloup N, Lössl P, Meijer DH, Brennich M, Heck AJR, Thies-Weesie DME, Janssen BJC
RgGuinier 3.7 nm
Dmax 13.5 nm
VolumePorod 253 nm3

SASDB55 – Glycosylated myelin-associated glycoprotein full extracellular domain (immunoglobulin domains 1-5)

Myelin-associated glycoprotein Ig domains 1-5 experimental SAS data
NONE model
Sample: Myelin-associated glycoprotein Ig domains 1-5 dimer, 108 kDa Mus musculus protein
Buffer: 25 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 6.8 nm
Dmax 23.8 nm
VolumePorod 177 nm3

SASDB26 – Glycosylated myelin-associated glycoprotein full extracellular domain (Ig1-5) I473E mutant

Myelin-associated glycoprotein (20-508; I473E mutant) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myelin-associated glycoprotein (20-508; I473E mutant) monomer, 54 kDa Mus musculus protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 11
Structural basis of myelin-associated glycoprotein adhesion and signalling. Nat Commun 7:13584 (2016)
Pronker MF, Lemstra S, Snijder J, Heck AJ, Thies-Weesie DM, Pasterkamp RJ, Janssen BJ
RgGuinier 6.0 nm
Dmax 20.6 nm
VolumePorod 117 nm3