Browse by ORGANISM: Mus musculus (Mouse)

SASDFS3 – Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D

Transcription intermediary factor 1-beta experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 7.0 nm
Dmax 23.2 nm
VolumePorod 232 nm3

SASDFT3 – Murine transcription intermediary factor 1-beta, TRIM28 RBCC assembly-null mutation R184D, complexed with the Krab domain of ZFP809 fused to an N-terminal MBP

Transcription intermediary factor 1-betaZinc finger protein 809 N-terminal MBP fusion experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta dimer, 82 kDa Mus musculus protein
Zinc finger protein 809 N-terminal MBP fusion monomer, 52 kDa Mus musculus protein
Buffer: 10 mM Tris 300 mM NaCl 0.1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Aug 10
A Dissection of Oligomerisation by the TRIM28 Tripartite Motif and the Interaction with Members of the Krab-ZFP Family. J Mol Biol (2019)
Sun Y, Keown JR, Black MM, Raclot C, Demarais N, Trono D, Turelli P, Goldstone DC
RgGuinier 6.4 nm
Dmax 22.0 nm
VolumePorod 252 nm3

SASDDQ4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 experimental SAS data
DAMMIN model
Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein
Insulin gene enhancer protein ISL-2 monomer, 4 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19
Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 20 nm3

SASDDR4 – LIM/homeobox protein Lhx4 LIM domains fused to the LIM interaction domain (LID) R282G mutant of Insulin gene enhancer protein ISL-2

LIM/homeobox protein Lhx4Insulin gene enhancer protein ISL-2 (R282G) experimental SAS data
DAMMIN model
Sample: LIM/homeobox protein Lhx4 monomer, 15 kDa Mus musculus protein
Insulin gene enhancer protein ISL-2 (R282G) monomer, 4 kDa Mus musculus protein
Buffer: 20 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 19
Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins (2019)
Stokes PH, Robertson NO, Silva AP, Estephan T, Trewhella J, Guss JM, Matthews JM
RgGuinier 2.3 nm
Dmax 8.5 nm
VolumePorod 21 nm3

SASDES5 – Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 (mmNT-SRCR3) - from SEC-SAXS

Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 monomer, 13 kDa Mus musculus protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 20
Structural characterization of the third scavenger receptor cysteine-rich domain of murine neurotrypsin. Protein Sci 28(4):746-755 (2019)
Canciani A, Catucci G, Forneris F
RgGuinier 1.5 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDDY2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig

Leucine-rich repeat and fibronectin type-III domain-containing protein 4 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 4 dimer, 79 kDa Mus musculus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 13
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 3.7 nm
Dmax 12.1 nm
VolumePorod 183 nm3

SASDDZ2 – Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn

Leucine-rich repeat and fibronectin type-III domain-containing protein 4 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 4: Fragment SALM3 LRR-Ig-Fn Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 4 dimer, 109 kDa Mus musculus protein
Buffer: 20 mM Tris HCl, 100 mM NaCl, 0.02% NaN3,, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Mar 11
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 4.8 nm
Dmax 17.1 nm
VolumePorod 313 nm3

SASDD23 – Leucine-rich repeat and fibronectin type-III domain-containing protein 5: Fragment SALM5 LRR-Ig

Leucine-rich repeat and fibronectin type-III domain-containing protein 5 experimental SAS data
Leucine-rich repeat and fibronectin type-III domain-containing protein 5: Fragment SALM5 LRR-Ig Rg histogram
Sample: Leucine-rich repeat and fibronectin type-III domain-containing protein 5 dimer, 82 kDa Mus musculus protein
Buffer: 30 mM Tris-Cl, 150 mM NaCl, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2016 Jun 8
The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition. Protein Eng Des Sel (2018)
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 155 nm3

SASDC99 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - 100% D2O SANS

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SANS data collected at NG7, NIST Center for High Resolution Neutron Scattering (CHRNS) on 2015 Nov 25
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 3.1 nm
Dmax 9.1 nm

SASDCA9 – Nanolipoprotein Particle (NLP) from In Vitro Assembly - SAXS

Apolipoprotein A-I1,2-dimyristoyl-sn-glycero-3-phosphocholine experimental SAS data
Apolipoprotein A-I 1,2-dimyristoyl-sn-glycero-3-phosphocholine Kratky plot
Sample: Apolipoprotein A-I dimer, 50 kDa Mus musculus protein
1,2-dimyristoyl-sn-glycero-3-phosphocholine, 92 kDa
Buffer: PBS in D2O, pH: 7.4
Experiment: SAXS data collected at SAXSLab Bio-Ganesha, Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology (IBBR/NIST) on 2015 Nov 24
Small-angle X-ray and neutron scattering demonstrates that cell-free expression produces properly formed disc-shaped nanolipoprotein particles. Protein Sci 27(3):780-789 (2018)
Cleveland TE 4th, He W, Evans AC, Fischer NO, Lau EY, Coleman MA, Butler P
RgGuinier 4.8 nm
Dmax 12.0 nm