Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDP62 – dark-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Orange carotenoid-binding protein experimental SAS data

Sample:	Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

Oligomerization processes limit photoactivation and recovery of the orange carotenoid protein. Biophys J 121(15):2849-2872 (2022)
Andreeva EA, Niziński S, Wilson A, Levantino M, De Zitter E, Munro R, Muzzopappa F, Thureau A, Zala N, Burdzinski G, Sliwa M, Kirilovsky D, Schirò G, Colletier JP

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	58	nm³

SASDP72 – dark-adapted Orange Carotenoid Protein-R27LNtag at 14 mg/mL

Sample:	Orange carotenoid-binding protein monomer, 35 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	2.3	nm
D_max	8.0	nm
Volume^Porod	58	nm³

SASDP82 – light-adapted Orange Carotenoid Protein-wtCtag at 0.7 mg/mL

Sample:	Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	2.9	nm
D_max	14.0	nm
Volume^Porod	63	nm³

SASDP92 – light-adapted Orange Carotenoid Protein-wtCtag at 3.5 mg/mL

Sample:	Orange carotenoid-binding protein dimer, 70 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	3.4	nm
D_max	17.0	nm
Volume^Porod	78	nm³

SASDPA2 – light-adapted Orange Carotenoid Protein-wtCtag at 10.5 mg/mL

Sample:	Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	4.4	nm
D_max	31.0	nm
Volume^Porod	140	nm³

SASDPB2 – light-adapted Orange Carotenoid Protein-R27LNtag at 0.7 mg/mL

Sample:	Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	3.3	nm
D_max	15.0	nm
Volume^Porod	70	nm³

SASDPC2 – light-adapted Orange Carotenoid Protein-R27LNtag at 3.5 mg/mL

Sample:	Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	3.4	nm
D_max	19.0	nm
Volume^Porod	70	nm³

SASDPD2 – light-adapted Orange Carotenoid Protein-R27LNtag at 10.5 mg/mL

Sample:	Orange carotenoid-binding protein, 105 kDa Synechocystis sp. (strain … protein
Buffer:	50 mM Tris, 150 mM NaCL, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2019 Mar 23

R_g^Guinier	4.8	nm
D_max	26.0	nm
Volume^Porod	120	nm³

SASDJE6 – Metallothionein (MT) 2 protein from the plant Cicer arietinum

Metallothionein-like protein 2 experimental SAS data

Metallothionein (MT) 2 protein from the plant Cicer arietinum Rg histogram

Sample:	Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	1.9	nm
D_max	7.3	nm
Volume^Porod	5	nm³

SASDJF6 – Apo-full-length protein titrated with Cd(II) ions

Apo-full-length protein titrated with Cd(II) ions Rg histogram

Sample:	Metallothionein-like protein 2 monomer, 8 kDa Cicer arietinum protein
Buffer:	10 mM Tris, 50 mM NaCl + 1mM TCEP (apoMT), pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2020 Aug 8

Structural Characterization of Plant Metallothionein
Dima Molodenskiy

R_g^Guinier	2.6	nm
D_max	10.0	nm
Volume^Porod	13	nm³

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