Small Angle Scattering Biological Data Bank SASBDB

Browse by ORGANISM: other species

SASDPS2 – apo-Thermus thermophilus argonaute protein at 10 °C

Piwi domain-containing protein experimental SAS data

Sample:	Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer:	20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7

Argonaute protein SAXS investigation
lirong zheng

R_g^Guinier	3.1	nm
D_max	9.7	nm
Volume^Porod	122	nm³

SASDPT2 – apo-Thermus thermophilus argonaute protein at 25 °C

Sample:	Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer:	20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7

Argonaute protein SAXS investigation
lirong zheng

R_g^Guinier	3.1	nm
D_max	9.7	nm
Volume^Porod	120	nm³

SASDPU2 – apo-Thermus thermophilus argonaute protein at 75 °C

Sample:	Piwi domain-containing protein monomer, 77 kDa Thermus thermophilus (strain … protein
Buffer:	20 mM Tris–HCl, 250 mM NaCl, 2mM DTT, pH: 8
Experiment:	SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2021 May 7

Argonaute protein SAXS investigation
lirong zheng

R_g^Guinier	3.3	nm
D_max	9.7	nm
Volume^Porod	175	nm³

SASDMS5 – Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion

RNA recognition motif (RRM)-containing protein 4 experimental SAS data

Glutathione S-transferase/RNA recognition motif (RRM)-containing protein 4 fusion Rg histogram

Sample:	RNA recognition motif (RRM)-containing protein 4 monomer, 111 kDa Ustilago maydis protein
Buffer:	20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 30

A MademoiseLLE domain binding platform links the key RNA transporter to endosomes PLOS Genetics 18(6):e1010269 (2022)
Devan S, Schott-Verdugo S, Müntjes K, Bismar L, Reiners J, Hachani E, Schmitt L, Höppner A, Smits S, Gohlke H, Feldbrügge M, Mitchell A

R_g^Guinier	8.8	nm
D_max	30.7	nm
Volume^Porod	587	nm³

SASDMT5 – N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4

RNA recognition motif (RRM)-containing protein 4 NT4 experimental SAS data

N-terminal histidine tagged RNA recognition motif (RRM)-containing protein 4 NT4 Rg histogram

Sample:	RNA recognition motif (RRM)-containing protein 4 NT4 monomer, 40 kDa Ustilago maydis protein
Buffer:	20 mM Hepes, 200 mM NaCl, 1 mM βME, pH: 8
Experiment:	SAXS data collected at BM29, ESRF on 2021 Apr 30

R_g^Guinier	5.6	nm
D_max	18.5	nm
Volume^Porod	123	nm³

SASDNU9 – Mycocerosic acid synthase in its apo form and at neutral pH

Mycocerosic acid synthase experimental SAS data

Sample:	Mycocerosic acid synthase monomer, 224 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Oct 1

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L

R_g^Guinier	5.7	nm
D_max	21.0	nm
Volume^Porod	420	nm³

SASDNV9 – Phenolpthiocerol synthase A of BCG at neutral pH

Phenolpthiocerol synthesis type-I polyketide synthase ppsA experimental SAS data

Sample:	Phenolpthiocerol synthesis type-I polyketide synthase ppsA monomer, 199 kDa Mycobacterium bovis (strain … protein
Buffer:	50 mM Tris-HCl, 500 mM NaCl, 2 mM EDTA, 10% glycerol, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Jun 14

R_g^Guinier	6.7	nm
D_max	20.2	nm
Volume^Porod	680	nm³

SASDNV5 – Botulinum Toxin E at acidic pH

Botulinum Toxin Serotype E, endonegative experimental SAS data

Sample:	Botulinum Toxin Serotype E, endonegative monomer, 144 kDa Clostridium botulinum protein
Buffer:	10 mM sodium acetate (20 mM ionic strength), pH: 4
Experiment:	SAXS data collected at B21, Diamond Light Source on 2019 Oct 6

Elucidation of critical pH-dependent structural changes in Botulinum Neurotoxin E. J Struct Biol :107876 (2022)
Lalaurie CJ, Splevins A, Barata TS, Bunting KA, Higazi DR, Zloh M, Spiteri VA, Perkins SJ, Dalby PA

R_g^Guinier	4.3	nm
D_max	15.2	nm
Volume^Porod	240	nm³

SASDMC4 – Plasmodium falciparum tyrosyl-tRNA synthetase

Tyrosine--tRNA ligase experimental SAS data

Sample:	Tyrosine--tRNA ligase dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4

Reaction hijacking of tyrosine tRNA synthetase as a new whole-of-life-cycle antimalarial strategy Science 376(6597):1074-1079 (2022)
Xie S, Metcalfe R, Dunn E, Morton C, Huang S, Puhalovich T, Du Y, Wittlin S, Nie S, Luth M, Ma L, Kim M, Pasaje C, Kumpornsin K, Giannangelo C, Houghton F, Churchyard A, Famodimu M, Barry D, Gillett D, Dey S, Kosasih C, Newman W, Niles J, Lee M, Baum J, Ottilie S, Winzeler E, Creek D, Williamson N, Parker M, Brand S, Langston S, Dick L, Griffin M, Gould A, Tilley L

R_g^Guinier	3.7	nm
D_max	12.1	nm
Volume^Porod	126	nm³

SASDMD4 – Plasmodium falciparum tyrosyl-tRNA synthetase, S234C mutant

Tyrosine--tRNA ligase (S234C) experimental SAS data

Sample:	Tyrosine--tRNA ligase (S234C) dimer, 87 kDa Plasmodium falciparum (isolate … protein
Buffer:	20 mM Tris-HCl, 150 mM NaCl, 0.5 mM TCEP, 0.1% sodium azide, pH: 7.4
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2021 Jun 4

R_g^Guinier	3.7	nm
D_max	12.0	nm
Volume^Porod	124	nm³

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