Browse by ORGANISM: other species

SASDMK2 – SWAXS Data of Lysozyme (Average of 0.9, 1.8, 2.5 and 5 mg/ml) Analyzed in the q range of 0.08-9.9 1/nm

Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM NaOAc pH 3.8, 150 mM NaCl, pH: 3.8
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 May 2
Visualizing how inclusion of higher reciprocal space in SWAXS data analysis improves shape restoration of biomolecules: case of lysozyme. J Biomol Struct Dyn :1-15 (2021)
Ashish
RgGuinier 1.4 nm
Dmax 4.2 nm

SASDMG2 – SWAXS data from Lysozyme Solution [Protein concentration 0.4 mg/ml] at 10 °C

Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM NaOAc pH 3.8, 150 mM NaCl, pH: 3.8
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 May 2
Visualizing how inclusion of higher reciprocal space in SWAXS data analysis improves shape restoration of biomolecules: case of lysozyme. J Biomol Struct Dyn :1-15 (2021)
Ashish
RgGuinier 1.5 nm
Dmax 4.6 nm

SASDML2 – SWAXS Data of Lysozyme (Average of 0.9, 1.8, 2.5 and 5 mg/ml) Analyzed in the q range of 0.08-17.2 1/nm

Lysozyme C experimental SAS data
DAMMIF model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM NaOAc pH 3.8, 150 mM NaCl, pH: 3.8
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 May 2
Visualizing how inclusion of higher reciprocal space in SWAXS data analysis improves shape restoration of biomolecules: case of lysozyme. J Biomol Struct Dyn :1-15 (2021)
Ashish
RgGuinier 1.4 nm
Dmax 4.2 nm

SASDGW5 – Suppressor of Fused from Drosophila

Suppressor of fused homolog experimental SAS data
CUSTOM IN-HOUSE model
Sample: Suppressor of fused homolog monomer, 53 kDa Drosophila melanogaster protein
Buffer: 50 mM bis-TRIS pH 5.5, 200 mM NaCl, 10% glycerol, pH: 5.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 7
Suppressor of Fused
Valerie Biou
RgGuinier 2.7 nm
Dmax 8.8 nm
VolumePorod 12 nm3

SASDBL9 – Putative DNA binding protein YeeF-CT from Bacillus subtilis (amino acids 528-669)

Putative DNA binding protein experimental SAS data
DAMMIN model
Sample: Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17
YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal
RgGuinier 2.7 nm
Dmax 9.5 nm
VolumePorod 55 nm3

SASDBM9 – YezG: a cognate immunity protein of YeeF from Bacillus subtilis

YezG, cognate immunity protein of YeeF experimental SAS data
DAMMIN model
Sample: YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 17
YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal
RgGuinier 2.1 nm
Dmax 6.1 nm
VolumePorod 46 nm3

SASDBN9 – Putative DNA binding protein YeeF-CT in complex with the cognate immunity protein YezG from Bacillus subtilis

Putative DNA binding proteinYezG, cognate immunity protein of YeeF experimental SAS data
DAMMIN model
Sample: Putative DNA binding protein dimer, 37 kDa Bacillus subtilis subsp. … protein
YezG, cognate immunity protein of YeeF monomer, 19 kDa Bacillus subtilis subsp. … protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR - Institute of Microbial Technology (IMTech) on 2016 Aug 18
YeeF, a polymorphic toxin from Bacillus subtilis, is a ribosomal RNase with promiscuous DNA binding property
Krishan Gopal
RgGuinier 4.0 nm
Dmax 14.1 nm
VolumePorod 115 nm3

SASDCR8 – Glucose isomerase

Xylose isomerase experimental SAS data
Xylose isomerase Kratky plot
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 100 mM HEPES, 1 mM MgCl2, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 21
A high flux setup for millisecond-scale small-angle X-ray scattering studies on macromolecular solutions
Clement Blanchet
RgGuinier 2.7 nm

SASDDW9 – PAS fold family protein mPAC-Δ132 in the dark state (mPAC-Δ132, dark, without adenosine-5'-[(α,β)-methyleno]triphosphate, ApCpp)

PAS fold family experimental SAS data
PAS fold family Kratky plot
Sample: PAS fold family dimer, 78 kDa Coleofasciculus chthonoplastes PCC … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11
MPAC Delta132
Robert Lindner
RgGuinier 4.4 nm
Dmax 15.9 nm
VolumePorod 132 nm3

SASDDX9 – PAS fold family protein mPAC-Δ132 with the addition of adenosine-5'-[(α,β)-methyleno]triphosphate in the dark state (mPAC-Δ132, dark + ApCpp)

PAS fold family experimental SAS data
OTHER model
Sample: PAS fold family dimer, 78 kDa Coleofasciculus chthonoplastes PCC … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 5 % w/v Glycerol, 1 mM ApCpp, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11
MPAC Delta132
Robert Lindner
RgGuinier 4.0 nm
Dmax 12.8 nm
VolumePorod 99 nm3