Browse by ORGANISM: other species

SASDW44 – Immunoglobulin G4 (pH 2, 0.2 M NaCl), SEC-SAXS data

humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) experimental SAS data
humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) Kratky plot
Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein
Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 238 nm3

SASDW54 – Immunoglobulin G1 (pH 2, 0.2 M NaCl), SEC-SAXS data

humanized immunoglobulin G1 monoclonal antibody (trastuzumab) experimental SAS data
humanized immunoglobulin G1 monoclonal antibody (trastuzumab) Kratky plot
Sample: Humanized immunoglobulin G1 monoclonal antibody (trastuzumab) monomer, 148 kDa Mouse/human protein
Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2023 May 20
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.2 nm
Dmax 12.9 nm
VolumePorod 240 nm3

SASDW64 – Immunoglobulin G4 (pH 7)

humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) experimental SAS data
humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) Kratky plot
Sample: Humanized immunoglobulin G4 monoclonal antibody (lebrikizumab) monomer, 148 kDa Mouse/human protein
Buffer: 10 mM sodium phosphate, pH: 7
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2024 Nov 19
IgG4 and IgG1 undergo common acid‐induced compaction into an alternatively folded state FEBS Letters (2025)
Imamura H, Honda S
RgGuinier 4.8 nm
Dmax 15.6 nm
VolumePorod 211 nm3

SASDNX6 – ASO1 DNA in the absence of target RNA

LNA-DNA-LNA experimental SAS data
LNA-DNA-LNA Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.3 nm
Dmax 4.0 nm
VolumePorod 4 nm3

SASDNY6 – ASO2 DNA in the absence of target RNA

MOE PS gapmers 3-8-3 experimental SAS data
DAMMIF model
Sample: MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.3 nm
VolumePorod 5 nm3

SASDNZ6 – ASO3 RNA in the absence of target RNA

oME ASO experimental SAS data
oME ASO Kratky plot
Sample: OME ASO monomer, 4 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.4 nm
Dmax 4.0 nm
VolumePorod 7 nm3

SASDN27 – ASO1 DNA in the presence target RNA

LNA-DNA-LNAPSCK9, 24mer ASO binding site experimental SAS data
LNA-DNA-LNA PSCK9, 24mer ASO binding site Kratky plot
Sample: LNA-DNA-LNA monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 15 nm3

SASDN37 – ASO2 DNA in the presence of target RNA

MOE PS gapmers 3-8-3PSCK9, 24mer ASO binding site experimental SAS data
DAMMIF model
Sample: MOE PS gapmers 3-8-3 monomer, 4 kDa DNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 2.1 nm
Dmax 6.4 nm
VolumePorod 16 nm3

SASDN47 – ASO3 RNA in the presence of target RNA

oME ASOPSCK9, 24mer ASO binding site experimental SAS data
oME ASO PSCK9, 24mer ASO binding site Kratky plot
Sample: OME ASO monomer, 4 kDa RNA
PSCK9, 24mer ASO binding site monomer, 8 kDa RNA
Buffer: phosphate buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2021 Oct 21
Application of enhanced biophysical strategies to determine ASO/target RNA binding affinity and kinetics
Michael Lerche
RgGuinier 1.9 nm
Dmax 6.2 nm
VolumePorod 16 nm3

SASDVT5 – Marchantia polymorpha Auxin Response Factor 3

Auxin response factor experimental SAS data
GASBOR model
Sample: Auxin response factor monomer, 46 kDa Marchantia polymorpha protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BL11 - NCD, ALBA on 2019 Dec 3
The structure and function of the DNA binding domain of class B MpARF2 share more traits with class A AtARF5 than to that of class B AtARF1. Structure (2025)
Crespo I, Malfois M, Rienstra J, Tarrés-Solé A, van den Berg W, Weijers D, Boer DR
RgGuinier 2.6 nm
Dmax 6.2 nm
VolumePorod 59 nm3