Small Angle Scattering Biological Data Bank SASBDB

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SASDY65 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (5 mg/ml)

Heat shock cognate 71 kDa protein experimental SAS data

Heat shock cognate 71 kDa protein Kratky plot

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.7	nm
D_max	24.7	nm
Volume^Porod	352	nm³

SASDY75 – Heat shock cognate 71 kDa protein (Hsc70) T204A apo (7 mg/ml)

Sample:	Heat shock cognate 71 kDa protein, 71 kDa protein
Buffer:	20 mM HEPES, 150 mM KCl, 10 mM M gCl2, 1mM TCEP, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2025 Mar 6

Advances on the large-scale bacterial production and stable phosphorylation of Hsc70 Protein Expression and Purification :106923 (2026)
Kley N, Hecht-Bucher M, Itzen A

R_g^Guinier	5.6	nm
D_max	25.0	nm
Volume^Porod	360	nm³

SASDWG8 – NTF2L domain of G3BP1 (residues 1-140) at pH 7.5, 150 mM NaCl

NTF2L domain of Ras GTPase-activating protein-binding protein 1 experimental SAS data

Sample:	NTF2L domain of Ras GTPase-activating protein-binding protein 1 dimer, 32 kDa protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7.5
Experiment:	SAXS data collected at B21, Diamond Light Source on 2023 May 2

SAXS reveals the molecular basis underlying pH-driven G3BP1 conformational dynamics: implications for stress granule formation

R_g^Guinier	2.0	nm
D_max	6.5	nm
Volume^Porod	40	nm³

SASDVV9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in H₂O

β-chitin nanofibers from squid pens experimental SAS data

β-chitin nanofibers from squid pens Kratky plot

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate. ACS Appl Mater Interfaces (2026)
Sørensen HV, Montserrat-Canals M, Coder A, Prévost S, Krueger S, Vaaje-Kolstad G, Bjerregaard-Andersen K, Lund R, Krengel U

SASDVW9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 20 % D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 20% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

SASDVX9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 42 % D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 42% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

SASDVY9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

SASDVZ9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 66 % D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 66% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

SASDW22 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 80 % D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 80% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

SASDW32 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 100% D₂O

Sample:	Β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 100% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

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