Small Angle Scattering Biological Data Bank SASBDB

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SASDLH3 – Truncated Thermoplasma E2 catalytic core

Regulatory protein E2 experimental SAS data

Sample:	Regulatory protein E2 trimer, 73 kDa Human papillomavirus type … protein
Buffer:	50 mM Tris ⁄ HCl, pH 8.8, 100 mM NaCl, pH: 8.8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 May 27

Why are the 2-oxoacid dehydrogenase complexes so large? Generation of an active trimeric complex Biochemical Journal 463(3):405-412 (2014)
Marrott N, Marshall J, Svergun D, Crennell S, Hough D, van den Elsen J, Danson M

R_g^Guinier	3.1	nm
D_max	11.0	nm
Volume^Porod	149	nm³

SASDAL5 – Clostridium difficile bacteriophage 27 endolysin dimer, CD27L

Clostridium difficile bacteriophage 27 endolysin experimental SAS data

Sample:	Clostridium difficile bacteriophage 27 endolysin dimer, 64 kDa Clostridioides difficile protein
Buffer:	20 mM HEPES 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 17

The CD27L and CTP1L endolysins targeting Clostridia contain a built-in trigger and release factor. PLoS Pathog 10(7):e1004228 (2014)
Dunne M, Mertens HD, Garefalaki V, Jeffries CM, Thompson A, Lemke EA, Svergun DI, Mayer MJ, Narbad A, Meijers R

R_g^Guinier	3.3	nm
D_max	10.6	nm
Volume^Porod	72	nm³

SASDAM5 – Clostridium difficile bacteriophage 27 endolysin dimer C238R mutant, CD27L-C238R

Clostridium difficile bacteriophage 27 endolysin C238R mutant experimental SAS data

Sample:	Clostridium difficile bacteriophage 27 endolysin C238R mutant dimer, 64 kDa Clostridioides difficile protein
Buffer:	20 mM HEPES 500 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Jun 21

R_g^Guinier	4.2	nm
D_max	14.7	nm
Volume^Porod	92	nm³

SASDAP4 – Chitinase 60 from Moritella marina

Chitinase 60 from Moritella marina Rg histogram

Sample:	Chitinase 60 monomer, 61 kDa Moritella marina protein
Buffer:	20 mM Tris 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 18

Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Acta Crystallogr D Biol Crystallogr 70(Pt 3):676-84 (2014)
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W

R_g^Guinier	3.2	nm
D_max	11.3	nm
Volume^Porod	75	nm³

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data

Sample:	Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	4.9	nm
D_max	15.0	nm
Volume^Porod	115	nm³

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data

Sample:	Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

R_g^Guinier	5.9	nm
D_max	17.0	nm
Volume^Porod	130	nm³

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data

Sample:	Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	56	nm³

SASDBJ4 – Wild-type LytA choline-binding domain

Lytic Amidase choline-binding domain experimental SAS data

Sample:	Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11

R_g^Guinier	3.3	nm
D_max	10.0	nm
Volume^Porod	49	nm³

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data

PsrP functional binding region Rg histogram

Sample:	Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer:	20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	2.3	nm
D_max	7.7	nm
Volume^Porod	37	nm³

SASDA35 – SeZinT-SeZnuA complex

High-affinity zinc transporter periplasmic componentZinc/cadmium-binding protein experimental SAS data

Sample:	High-affinity zinc transporter periplasmic component monomer, 33 kDa Salmonella enterica subsp. … protein Zinc/cadmium-binding protein monomer, 23 kDa Salmonella enterica subsp. … protein
Buffer:	50 mM HEPES 50 mM KCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 May 9

The Salmonella enterica ZinT structure, zinc affinity and interaction with the high-affinity uptake protein ZnuA provide insight into the management of periplasmic zinc. Biochim Biophys Acta 1840(1):535-44 (2014)
Ilari A, Alaleona F, Tria G, Petrarca P, Battistoni A, Zamparelli C, Verzili D, Falconi M, Chiancone E

R_g^Guinier	2.5	nm
D_max	8.5	nm
Volume^Porod	55	nm³

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