Browse by ORGANISM: other species

SASDQP6 – Nanodisc with POPC and circularized membrane scaffolding protein (csMSP1E3D1)

circularized Membrane scaffolding protein 1 E3 D11-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Circularized Membrane scaffolding protein 1 E3 D1, 62 kDa protein
1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) None, lipid
Buffer: 20 mM Tris-HCl pH 7.5, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 5
Circularized and solubility‐enhanced MSP s facilitate simple and high‐yield production of stable nanodiscs for studies of membrane proteins in solution The FEBS Journal 286(9):1734-1751 (2019)
Johansen N, Tidemand F, Nguyen T, Rand K, Pedersen M, Arleth L
RgGuinier 5.8 nm
Dmax 14.5 nm

SASDE78 – Solution structure of the diadenylate cyclase/phosphoglucosamine mutase (DacA/GlmM) complex from Staphylococcus aureus

Diadenylate cyclasePhosphoglucosamine mutase experimental SAS data
DAMFILT model
Sample: Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein
Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A
RgGuinier 3.9 nm
Dmax 12.1 nm
VolumePorod 204 nm3

SASDE88 – Solution structure of phosphoglucosamine mutase (GlmM) from Staphylococcus aureus

Phosphoglucosamine mutase experimental SAS data
DAMFILT model
Sample: Phosphoglucosamine mutase dimer, 99 kDa Staphylococcus aureus protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A
RgGuinier 3.7 nm
Dmax 12.5 nm
VolumePorod 134 nm3

SASDE98 – Solution structure of Diadenylate cyclase (DacA) from Staphylococcus aureus

Diadenylate cyclase experimental SAS data
DAMFILT model
Sample: Diadenylate cyclase dimer, 39 kDa Staphylococcus aureus protein
Buffer: 30 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 May 7
Inhibition of the Staphylococcus aureus c-di-AMP cyclase DacA by direct interaction with the phosphoglucosamine mutase GlmM. PLoS Pathog 15(1):e1007537 (2019)
Tosi T, Hoshiga F, Millership C, Singh R, Eldrid C, Patin D, Mengin-Lecreulx D, Thalassinos K, Freemont P, Gründling A
RgGuinier 2.6 nm
Dmax 8.6 nm
VolumePorod 57 nm3

SASDDJ6 – SAM-dependent O-Methyltransferase from Mycobacterium hassiacum

Methyltransferase domain protein experimental SAS data
MODELLER model
Sample: Methyltransferase domain protein dimer, 51 kDa Mycolicibacterium hassiacum protein
Buffer: 20 mM bis-tris propane, 50 mM NaCl, 2 mM DTT, 5% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jun 21
Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides. Proc Natl Acad Sci U S A 116(3):835-844 (2019)
Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N
RgGuinier 2.6 nm
Dmax 8.5 nm
VolumePorod 86 nm3

SASDEY3 – Tryparedoxin, reduced state

Tryparedoxin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.8 nm
VolumePorod 27 nm3

SASDEZ3 – Tryparedoxin, oxidized state

Tryparedoxin experimental SAS data
GASBOR model
Sample: Tryparedoxin monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 6.5 nm
VolumePorod 27 nm3

SASDE24 – Tryparedoxin, in the presence of inhibitor CFT (2-(chloromethyl)-5-(4-fluorophenyl)thieno[2,3-d]pyrimidin-4(3H)-one)

Tryparedoxin experimental SAS data
SASREF MX model
Sample: Tryparedoxin, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 2.0 nm
Dmax 6.9 nm
VolumePorod 45 nm3

SASDE34 – Tryparedoxin W39A, reduced state

Tryparedoxin W39A experimental SAS data
OTHER model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3

SASDE44 – Tryparedoxin W39A, oxidized state

Tryparedoxin W39A experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tryparedoxin W39A monomer, 16 kDa Trypanosoma brucei brucei protein
Buffer: 10 mM HEPES pH 7.5, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 May 31
Inhibitor-induced dimerization of an essential oxidoreductase from African Trypanosomes. Angew Chem Int Ed Engl (2019)
Wagner A, Le TA, Brennich M, Klein P, Bader N, Diehl E, Paszek D, Weickhmann AK, Dirdjaja N, Krauth-Siegel RL, Engels B, Opatz T, Schindelin H, Hellmich UA
RgGuinier 1.6 nm
Dmax 5.5 nm
VolumePorod 23 nm3