Small Angle Scattering Biological Data Bank SASBDB

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SASDE58 – Complex of toxin GraT with antotoxin GraA

Putative killer protein, Toxin GraT.antitoxin GraA, antidote of toxin GraTPutative killer protein, Toxin GraT experimental SAS data

Putative killer protein, Toxin GraT. antitoxin GraA, antidote of toxin GraT Putative killer protein, Toxin GraT Kratky plot

Sample:	Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein Putative killer protein, Toxin GraT monomer, 11 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at B21, Diamond Light Source on 2018 Jun 29

A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT. Nat Commun 10(1):972 (2019)
Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R

R_g^Guinier	2.3	nm
D_max	7.9	nm
Volume^Porod	63	nm³

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator

GraTA operator regionantitoxin GraA, antidote of toxin GraTatitoxin GraA, antidote of GraT. experimental SAS data

GraTA operator region antitoxin GraA, antidote of toxin GraT atitoxin GraA, antidote of GraT. Kratky plot

Sample:	GraTA operator region monomer, 20 kDa DNA Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein Atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein
Buffer:	50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2017 Dec 17

R_g^Guinier	2.9	nm
D_max	10.3	nm
Volume^Porod	94	nm³

SASDDG5 – Mammalian prion protein mRNA (PrP mRNA wild type)

octo-repeat PrP mRNA experimental SAS data

Sample:	Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Nov 4

Octa-repeat domain of the mammalian prion protein mRNA forms stable A-helical hairpin structure rather than G-quadruplexes. Sci Rep 9(1):2465 (2019)
Czech A, Konarev PV, Goebel I, Svergun DI, Wills PR, Ignatova Z

R_g^Guinier	7.4	nm
D_max	25.0	nm
Volume^Porod	165	nm³

SASDDH5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl

Sample:	Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	8.8	nm
D_max	31.0	nm
Volume^Porod	240	nm³

SASDDJ5 – Mammalian prion protein mRNA (PrP mRNA wild type) with LiCl

Sample:	Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM LiCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	9.0	nm
D_max	31.0	nm
Volume^Porod	225	nm³

SASDDK5 – Mammalian prion protein mRNA (PrP mRNA wild type) with KCl and pyridostatin (PDS)

Sample:	Octo-repeat PrP mRNA dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	9.1	nm
D_max	31.0	nm
Volume^Porod	220	nm³

SASDDL5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA)

octo-repeat PrP mRNA mutant experimental SAS data

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	7.0	nm
D_max	24.0	nm
Volume^Porod	150	nm³

SASDDM5 – Mutant mammalian prion protein mRNA (octo-repeat PrP mRNA) with KCl

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	9.0	nm
D_max	33.0	nm
Volume^Porod	210	nm³

SASDDN5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with LiCl

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM LiCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	8.9	nm
D_max	33.0	nm
Volume^Porod	223	nm³

SASDDP5 – Mutant mammalian prion protein mRNA (octo-repear PrP mRNA) with KCl and pyridostatin (PDS)

Sample:	Octo-repeat PrP mRNA mutant dimer, 144 kDa human PrP ORF RNA
Buffer:	10 mM Tris buffer with 100 mM KCl and 1 mM PDS, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Jun 6

R_g^Guinier	9.2	nm
D_max	33.0	nm
Volume^Porod	260	nm³

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