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SASDDR6 – The ferredoxin protease, FusC, E83A mutant

Ferredoxin protease E83A mutant experimental SAS data
Ferredoxin protease E83A mutant Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 12.7 nm
VolumePorod 152 nm3

SASDDU6 – The ferredoxin protease, FusC, E83A mutant

Ferredoxin protease E83A mutant experimental SAS data
Ferredoxin protease E83A mutant Kratky plot
Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein
Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26
FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. PLoS Biol 16(8):e2006026 (2018)
Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
RgGuinier 3.7 nm
Dmax 13.8 nm
VolumePorod 152 nm3

SASDEM4 – HrpG/HrpV/HrpJ gatekeeper complex from Erwinia amylovora

Type III secretion protein HrpGType III secretion protein HrpVHypersensitivity response secretion protein HrpJ experimental SAS data
CORAL model
Sample: Type III secretion protein HrpG, 19 kDa Erwinia amylovora protein
Type III secretion protein HrpV, 13 kDa Erwinia amylovora protein
Hypersensitivity response secretion protein HrpJ, 42 kDa Erwinia amylovora protein
Buffer: 20mM Tris-Cl, pH 8.0, 100mM NaCl, 2mM DTT, 0.5mM EDTA, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Apr 3
Migration of Type III Secretion System Transcriptional Regulators Links Gene Expression to Secretion. MBio 9(4) (2018)
Charova SN, Gazi AD, Mylonas E, Pozidis C, Sabarit B, Anagnostou D, Psatha K, Aivaliotis M, Beuzon CR, Panopoulos NJ, Kokkinidis M
RgGuinier 4.0 nm
Dmax 16.0 nm

SASDDZ8 – Designed hollow protein nanoparticle TIP60 (Truncated Icosahedral Protein composed of 60-mer fusion proteins)

Fusion protein of LSm and MyoX-coil experimental SAS data
DAMMIN model
Sample: Fusion protein of LSm and MyoX-coil, 1066 kDa Artificial protein protein
Buffer: 25 mM HEPES, 100 mM NaCl, 1 mM EDTA, 5% glycerol, pH: 8
Experiment: SAXS data collected at BL-6A, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Nov 11
Design of Hollow Protein Nanoparticles with Modifiable Interior and Exterior Surfaces. Angew Chem Int Ed Engl (2018)
Kawakami N, Kondo H, Matsuzawa Y, Hayasaka K, Nasu E, Sasahara K, Arai R, Miyamoto K
RgGuinier 9.4 nm
Dmax 23.0 nm

SASDDV8 – Boiled chicken egg albumen

Ovalbumin experimental SAS data
Ovalbumin Kratky plot
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDW8 – Chinese century egg albumen (pidan) made from quail egg

Ovalbumin (common quail) experimental SAS data
Ovalbumin (common quail) Kratky plot
Sample: Ovalbumin (common quail) monomer, 42 kDa Coturnix coturnix protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDX8 – Raw chicken egg albumen

Ovalbumin experimental SAS data
Ovalbumin Kratky plot
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: Water, pH: 7
Experiment: SAXS data collected at Bruker Nonius FR591, University of Pennslyvania on 2013 Jun 27
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDY8 – Chicken ovalbumin gel at high pH

Ovalbumin experimental SAS data
DAMMIF model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: 0.16 M NaOH, pH: 13.2
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2014 Feb 21
The Proof Is in the Pidan: Generalizing Proteins as Patchy Particles. ACS Cent Sci 4(7):840-853 (2018)
Cai J, Sweeney AM

SASDDF7 – Armless mitochondrial tRNA-Arg from Romanomermis culicivorax

Arginyl transfer RNA experimental SAS data
PYMOL model
Sample: Arginyl transfer RNA monomer, 13 kDa Romanomermis culicivorax RNA
Buffer: 50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Dec 16
Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax. Nucleic Acids Res (2018)
Jühling T, Duchardt-Ferner E, Bonin S, Wöhnert J, Pütz J, Florentz C, Betat H, Sauter C, Mörl M
RgGuinier 2.1 nm
Dmax 6.7 nm
VolumePorod 20 nm3

SASDDG7 – Armless mitochondrial tRNA-Ile from Romanomermis culicivorax

Isoleucyl transfer RNA experimental SAS data
PYMOL model
Sample: Isoleucyl transfer RNA monomer, 15 kDa Romanomermis culicivorax RNA
Buffer: 50 mM HEPES-NaOH 10 mM MgCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Dec 16
Small but large enough: structural properties of armless mitochondrial tRNAs from the nematode Romanomermis culicivorax. Nucleic Acids Res (2018)
Jühling T, Duchardt-Ferner E, Bonin S, Wöhnert J, Pütz J, Florentz C, Betat H, Sauter C, Mörl M
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 20 nm3