|
|
|
Sample: |
S-crystallin, Q25367_DOROP dimer, 55 kDa Doryteuthis pealeii protein
|
Buffer: |
PBS, phosphate buffered saline, pH: 7 |
Experiment: |
SAXS
data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Nov 12
|
Eye patches: Protein assembly of index-gradient squid lenses.
Science 357(6351):564-569 (2017)
Cai J, Townsend JP, Dodson TC, Heiney PA, Sweeney AM
|
|
|
|
|
|
Sample: |
Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
|
Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Mar 16
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
RgGuinier |
2.5 |
nm |
Dmax |
11.5 |
nm |
VolumePorod |
17 |
nm3 |
|
|
|
|
|
Sample: |
Cold shock-like protein monomer, 7 kDa Thermotoga maritima protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
|
Buffer: |
PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jun 15
|
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements.
Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
|
RgGuinier |
2.2 |
nm |
Dmax |
8.3 |
nm |
VolumePorod |
9 |
nm3 |
|
|
|
|
|
Sample: |
Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
|
Buffer: |
10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
|
Structural characterization of bacterial collagenases
University of Arkansas PhD thesis 2431 (2017)
Bauer R
|
RgGuinier |
2.4 |
nm |
Dmax |
7.5 |
nm |
VolumePorod |
36 |
nm3 |
|
|
|
|
|
Sample: |
Class1 collagenase collagen-binding domain monomer, 27 kDa Hathewaya histolytica protein
|
Buffer: |
10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
|
Structural characterization of bacterial collagenases
University of Arkansas PhD thesis 2431 (2017)
Bauer R
|
RgGuinier |
2.4 |
nm |
Dmax |
9.8 |
nm |
VolumePorod |
33 |
nm3 |
|
|
|
|
|
Sample: |
Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
|
Buffer: |
10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Sep 14
|
Structural characterization of bacterial collagenases
University of Arkansas PhD thesis 2431 (2017)
Bauer R
|
RgGuinier |
2.5 |
nm |
Dmax |
9.4 |
nm |
VolumePorod |
36 |
nm3 |
|
|
|
|
|
Sample: |
Class1 collagenase monomer, 27 kDa Hathewaya histolytica protein
|
Buffer: |
10 mM HEPES 100 mM NaCL, 2% glycerol, pH: 7.5 |
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Sep 14
|
Structural characterization of bacterial collagenases
University of Arkansas PhD thesis 2431 (2017)
Bauer R
|
RgGuinier |
2.6 |
nm |
Dmax |
12.8 |
nm |
VolumePorod |
33 |
nm3 |
|
|
|
|
|
Sample: |
Major viral transcription factor ICP4 dimer, 49 kDa Human alphaherpesvirus 1 protein
Immediate Early 3 dimer, 12 kDa DNA
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.4 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2015 Jul 27
|
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
|
RgGuinier |
2.5 |
nm |
Dmax |
8.3 |
nm |
VolumePorod |
90 |
nm3 |
|
|
|
|
|
Sample: |
Major viral transcription factor ICP4 dimer, 49 kDa Human alphaherpesvirus 1 protein
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.4 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2015 Jul 27
|
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
|
RgGuinier |
2.9 |
nm |
Dmax |
12.7 |
nm |
|
|
|
|
|
Sample: |
Immediate Early 3 dimer, 12 kDa DNA
|
Buffer: |
20 mM HEPES, 150 mM NaCl, pH: 7.4 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2015 Nov 12
|
The herpes viral transcription factor ICP4 forms a novel DNA recognition complex.
Nucleic Acids Res 45(13):8064-8078 (2017)
Tunnicliffe RB, Lockhart-Cairns MP, Levy C, Mould AP, Jowitt TA, Sito H, Baldock C, Sandri-Goldin RM, Golovanov AP
|
RgGuinier |
1.9 |
nm |
Dmax |
6.9 |
nm |
|
|