Small Angle Scattering Biological Data Bank SASBDB

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SASDCW9 – Bacteriophage phi-X174 - ~245s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data

Sample:	Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer:	0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG

R_g^Guinier

12.9

SASDCX9 – Bacteriophage phi-X174 - ~545s after mixing with LPS

Sample:	Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer:	0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25

R_g^Guinier

12.9

SASDBY4 – Pentameric Nucleoplasmin-histone H2A/H2B complex

Nucleoplasmin core + A2Histone H2A (ΔAla127)Histone H2B 1.1 (Ser33Thr) experimental SAS data

Sample:	Nucleoplasmin core + A2 pentamer, 81 kDa Xenopus laevis protein Histone H2A (ΔAla127) pentamer, 69 kDa Xenopus laevis protein Histone H2B 1.1 (Ser33Thr) pentamer, 67 kDa Xenopus laevis protein
Buffer:	20 mM Tris. 150 mM NaCl, 1 mM EDTA, 5 mM DTT, pH: 8
Experiment:	SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2016 Jan 7

Dynamic intramolecular regulation of the histone chaperone nucleoplasmin controls histone binding and release. Nat Commun 8(1):2215 (2017)
Warren C, Matsui T, Karp JM, Onikubo T, Cahill S, Brenowitz M, Cowburn D, Girvin M, Shechter D

R_g^Guinier	4.4	nm
D_max	14.0	nm
Volume^Porod	402	nm³

SASDCB7 – Streptococcus pneumoniae phosphodiesterase Pde2

DHH subfamily 1 protein experimental SAS data

Sample:	DHH subfamily 1 protein dimer, 70 kDa Streptococcus pneumoniae serotype … protein
Buffer:	20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23

Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima. Structure 25(12):1887-1897.e4 (2017)
Drexler DJ, Müller M, Rojas-Cordova CA, Bandera AM, Witte G

R_g^Guinier	2.7	nm
D_max	7.7	nm
Volume^Porod	87	nm³

SASDCC7 – Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)

Sample:	T.maritima PDE dimer, 76 kDa Thermotoga maritima protein
Buffer:	25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 17

R_g^Guinier	2.8	nm
D_max	7.9	nm
Volume^Porod	115	nm³

SASDCD7 – Thermotoga maritima phosphodiesterase TM1595 (wildtype, TmPde)

Thermotoga maritima phosphodiesterase (wildtype, TmPDE, TM1595) experimental SAS data

Sample:	Thermotoga maritima phosphodiesterase (wildtype, TmPDE, TM1595) dimer, 76 kDa Thermotoga maritima protein
Buffer:	20mM Tris, 200 mM NaCl, 5%(v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Jun 23

R_g^Guinier	2.7	nm
D_max	7.8	nm
Volume^Porod	106	nm³

SASDB89 – Telomeric DNA duplex

telomere DNA duplex experimental SAS data

Sample:	Telomere DNA duplex monomer, 11 kDa DNA
Buffer:	20 mM Tris-HCl, 50 mM LiCl, pH: 7.5
Experiment:	SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2016 May 3

Basic domain of telomere guardian TRF2 reduces D-loop unwinding whereas Rap1 restores it. Nucleic Acids Res 45(21):12170-12180 (2017)
Necasová I, Janoušková E, Klumpler T, Hofr C

R_g^Guinier	1.6	nm
D_max	5.7	nm
Volume^Porod	12	nm³

SASDCK9 – Catalytic domain (AC) of B. Pertussis Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data

Sample:	Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment:	SAXS data collected at SWING, SOLEIL on 2015 Jun 19

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A

R_g^Guinier	2.6	nm
D_max	9.1	nm
Volume^Porod	60	nm³

SASDBJ7 – Pomacea maculata perivitellin 1

Pomacea maculata perivitellin 1 experimental SAS data

Sample:	Pomacea maculata perivitellin 1 dodecamer, 278 kDa Pomacea maculata protein
Buffer:	100 mM Phosphate Buffer, pH: 7.4
Experiment:	SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2015 Mar 26

Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins. Sci Rep 7(1):15848 (2017)
Pasquevich MY, Dreon MS, Qiu JW, Mu H, Heras H

R_g^Guinier	4.2	nm
D_max	14.3	nm
Volume^Porod	537	nm³

SASDCN5 – Vaccinia virus DNA polymerase

Sample:	DNA polymerase E9 monomer, 117 kDa Vaccinia virus protein
Buffer:	20 mM Tris HCl, 100 mM NaCl, 4 mM DTT, pH: 7
Experiment:	SAXS data collected at BM29, ESRF on 2014 Nov 27

The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding. Nat Commun 8(1):1455 (2017)
Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F

R_g^Guinier	3.9	nm
D_max	12.0	nm
Volume^Porod	202	nm³

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