|
|
|
Sample: |
Functional binding region (120-395) of the pneumococcal serine-rich repeat protein monomer, 30 kDa Streptococcus pneumoniae protein
|
Buffer: |
PBS 5 % Glycerol, pH: 7.4 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2013 Feb 27
|
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation.
Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
|
RgGuinier |
2.9 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
41 |
nm3 |
|
|
|
|
|
Sample: |
DNA 22mer monomer, 12 kDa DNA
|
Buffer: |
20 mM Tris-HCl 150 mM NaCl 5% glycerol 1 mM DTT, pH: 7.4 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Dec 6
|
The flexibility of a homeodomain transcription factor heterodimer and its allosteric regulation by DNA binding.
FEBS J 283(16):3134-54 (2016)
Mathiasen L, Valentini E, Boivin S, Cattaneo A, Blasi F, Svergun DI, Bruckmann C
|
RgGuinier |
2.2 |
nm |
Dmax |
7.5 |
nm |
VolumePorod |
19 |
nm3 |
|
|
|
|
|
Sample: |
Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
|
Buffer: |
50 mM Tris 50 mM boric acid 1 mM EDTA, pH: 8 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2014 Nov 6
|
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
|
RgGuinier |
3.4 |
nm |
Dmax |
12.6 |
nm |
VolumePorod |
115 |
nm3 |
|
|
|
|
|
Sample: |
Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
|
Buffer: |
10 mM Tris 300 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2014 Nov 6
|
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
|
RgGuinier |
3.9 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
121 |
nm3 |
|
|
|
|
|
Sample: |
Aureochrome 1a bZIP-LOV module dimer, 57 kDa Phaeodactylum tricornutum protein
|
Buffer: |
10 mM Tris 300 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2014 Nov 6
|
Allosteric communication between DNA-binding and light-responsive domains of diatom class I aureochromes.
Nucleic Acids Res 44(12):5957-70 (2016)
Banerjee A, Herman E, Serif M, Maestre-Reyna M, Hepp S, Pokorny R, Kroth PG, Essen LO, Kottke T
|
RgGuinier |
3.8 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
117 |
nm3 |
|
|
|
|
|
Sample: |
Short-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 14 kDa Shewanella putrefaciens RNA
Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
Trimeric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821) trimer, 112 kDa Shewanella putrefaciens protein
Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
|
Buffer: |
50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2015 Jun 27
|
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system.
Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
|
RgGuinier |
4.1 |
nm |
Dmax |
14.2 |
nm |
|
|
|
|
|
Sample: |
Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
Hexameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821) hexamer, 223 kDa Shewanella putrefaciens protein
Wildtype-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 19 kDa RNA
|
Buffer: |
50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2015 Jun 27
|
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system.
Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
|
RgGuinier |
5.4 |
nm |
Dmax |
18.4 |
nm |
|
|
|
|
|
Sample: |
Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
Nonameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821) nonamer, 335 kDa Shewanella putrefaciens protein
Long-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 25 kDa RNA
|
Buffer: |
50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2016 Jan 30
|
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system.
Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
|
RgGuinier |
6.5 |
nm |
Dmax |
21.6 |
nm |
|
|
|
|
|
Sample: |
MVA F1L antiapoptotic Bcl-2 viral protein dimer, 51 kDa Vaccinia virus protein
|
Buffer: |
25 mM HEPES 150 mM NaCl 5 mM DTT, pH: 7.5 |
Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 15
|
The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation.
J Biol Chem 291(28):14600-8 (2016)
Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M
|
RgGuinier |
3.4 |
nm |
Dmax |
16.2 |
nm |
VolumePorod |
74 |
nm3 |
|
|
|
|
|
Sample: |
Stress-induced protein sti1 monomer, 62 kDa Leishmania braziliensis protein
|
Buffer: |
25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5 |
Experiment: |
SAXS
data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
|
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis.
Arch Biochem Biophys 600:12-22 (2016)
Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC
|
RgGuinier |
4.5 |
nm |
Dmax |
18.0 |
nm |
VolumePorod |
94 |
nm3 |
|
|