Browse by ORGANISM: other species

SASDD63 – Collagenase ColG s2s3as3b at pCa 3 (second analysis)

Collagenase ColG segement s2s3as3b experimental SAS data
DAMFILT model
Sample: Collagenase ColG segement s2s3as3b monomer, 37 kDa Hathewaya histolytica protein
Buffer: 10mM HEPES 100mM NaCl 0.2mM EGTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Oct 11
Ca2+ - Induced Structural Change of Multi-Domain Collagen Binding Segments of Collagenases ColG and ColH from Hathewaya histolytica University of Arkansas Dissertation - (2018)
Christopher E Ruth
RgGuinier 3.0 nm
Dmax 14.4 nm
VolumePorod 61 nm3

SASDDE3 – Human respiratory syncytial virus (HRSV) M2–1 RNA-binding core domain

Human respiratory syncytial virus M2-1 experimental SAS data
DAMMIF model
Sample: Human respiratory syncytial virus M2-1 monomer, 14 kDa Human orthopneumovirus protein
Buffer: 20 mM Tris–HCl, 300 mM NaCl,, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 13
Structure and stability of the Human respiratory syncytial virus M2-1 RNA-binding core domain reveals a compact and cooperative folding unit. Acta Crystallogr F Struct Biol Commun 74(Pt 1):23-30 (2018)
Molina IG, Josts I, Almeida Hernandez Y, Esperante S, Salgueiro M, Garcia Alai MM, de Prat-Gay G, Tidow H
RgGuinier 2.0 nm
Dmax 7.9 nm
VolumePorod 3 nm3

SASDDE6 – Photoreceptor sensory box 1 light-state (SB1-LOV-R66I)

Sensory box protein light-state (R66I) experimental SAS data
Photoreceptor sensory box 1 light-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein light-state (R66I) dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 9.2 nm
VolumePorod 58 nm3

SASDDG6 – Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I)

Sensory box protein dark-state experimental SAS data
Photoreceptor sensory box 1 dark-state (SB1-LOV-R66I) Rg histogram
Sample: Sensory box protein dark-state dimer, 37 kDa Pseudomonas putida protein
Buffer: 10mM Tris, 10 mM NaCl, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Dec 2
Small-angle X-ray scattering study of the kinetics of light-dark transition in a LOV protein. PLoS One 13(7):e0200746 (2018)
Röllen K, Granzin J, Batra-Safferling R, Stadler AM
RgGuinier 2.6 nm
Dmax 8.0 nm
VolumePorod 57 nm3

SASDDK7 – Disrupted-in-Schizophrenia 1 protein (DISC1 12d2)

Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 experimental SAS data
GASBOR model
Sample: Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 1
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 41 nm3

SASDDL7 – anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5 experimental SAS data
GASBOR model
Sample: Anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein
Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 1.8 nm
Dmax 8.4 nm
VolumePorod 21 nm3

SASDDM7 – Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 experimental SAS data
DAMMIF model
Sample: Anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein
Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19
Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein. PLoS One 13(1):e0191162 (2018)
Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
RgGuinier 3.1 nm
Dmax 10.4 nm
VolumePorod 77 nm3

SASDCT9 – Bacteriophage phi-X174 - Empty Capsid

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 14.0 nm

SASDCU9 – Bacteriophage phi-X174 - Before Reaction with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.1 nm

SASDCV9 – Bacteriophage phi-X174 - ~45s after mixing with LPS

Bacteriophage phi-X174 experimental SAS data
Bacteriophage phi-X174 Kratky plot
Sample: Bacteriophage phi-X174 monomer, 0 kDa protein
Buffer: 0.15 mg/mL LPS, 0.06 M NH4Cl2, 0.09 M NaCl, 0.1 M KCl, 1 mM MgS04, 1 mM CaCl2, 0.1 M Tris-HCl, pH: 7.4
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 25
Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls. Proc Natl Acad Sci U S A 114(52):13708-13713 (2017)
Sun Y, Roznowski AP, Tokuda JM, Klose T, Mauney A, Pollack L, Fane BA, Rossmann MG
RgGuinier 12.7 nm