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SASDDP2 – Sinorhizobium meliloti Proline Utilization A (PutA) at high concentration, 4.00 mg/ml

Sinorhizobium meliloti (SmPutA) experimental SAS data
MES-FOXS model
Sample: Sinorhizobium meliloti (SmPutA) monomer, 132 kDa Sinorhizobium meliloti protein
Buffer: 50 mM Tris, 1% (v/v) glycerol, 0.5 mM THP, and 50 mM NaCl, pH: 7.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Mar 27
Structures of Proline Utilization A (PutA) Reveal the Fold and Functions of the Aldehyde Dehydrogenase Superfamily Domain of Unknown Function. J Biol Chem 291(46):24065-24075 (2016)
Luo M, Gamage TT, Arentson BW, Schlasner KN, Becker DF, Tanner JJ
RgGuinier 3.9 nm
Dmax 11.9 nm
VolumePorod 277 nm3

SASDA89 – Complex between ovine GM-CSF and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorGranulocyte-macrophage colony-stimulating factor experimental SAS data
NONE model
Sample: GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein
Granulocyte-macrophage colony-stimulating factor dimer, 29 kDa Ovis aries protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Sep 10
Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF. Nat Commun 7:13228 (2016)
Felix J, Kandiah E, De Munck S, Bloch Y, van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN
RgGuinier 3.8 nm
Dmax 12.4 nm
VolumePorod 231 nm3

SASDA99 – Complex between ovine IL-2 and GM-CSF/IL-2 inhibition factor

GM-CSF/IL-2 inhibition factorInterleukin-2 experimental SAS data
NONE model
Sample: GM-CSF/IL-2 inhibition factor tetramer, 120 kDa Orf virus protein
Interleukin-2 monomer, 16 kDa Ovis aries protein
Buffer: 20 mM HEPES 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Sep 10
Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF. Nat Commun 7:13228 (2016)
Felix J, Kandiah E, De Munck S, Bloch Y, van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN
RgGuinier 4.1 nm
Dmax 12.9 nm
VolumePorod 253 nm3

SASDDD9 – Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain

cardiac myosin binding protein C: tri-helix bundle-C2 experimental SAS data
Cardiac myosin binding protein C: tri-helix bundle from the motif with C2 domain Rg histogram
Sample: Cardiac myosin binding protein C: tri-helix bundle-C2 monomer, 15 kDa human sequence obtained … protein
Buffer: 150 mM NaCl, 10 mM MES, 2 mM TCEP, 1 mM NaN3 at 4°C, pH: 6.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2015 Apr 18
A Highly Conserved Yet Flexible Linker Is Part of a Polymorphic Protein-Binding Domain in Myosin-Binding Protein C. Structure 24(11):2000-2007 (2016)
Michie KA, Kwan AH, Tung CS, Guss JM, Trewhella J
RgGuinier 1.9 nm
Dmax 8.0 nm
VolumePorod 20 nm3

SASDAT7 – Aptamer AIR-3 5FU

AIR3-5FU experimental SAS data
DAMMIF model
Sample: AIR3-5FU dimer, 69 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 77 nm3

SASDAU7 – Aptamer AIR-3 FdU

AIR3-FdU experimental SAS data
DAMMIF model
Sample: AIR3-FdU dimer, 69 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 5.0 nm
Dmax 16.0 nm
VolumePorod 67 nm3

SASDAV7 – Aptamer AIR-3A

AIR-3A experimental SAS data
DAMMIN model
Sample: AIR-3A dimer, 13 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 1.9 nm
Dmax 6.5 nm
VolumePorod 14 nm3

SASDAW7 – Aptamer AIR-3A 2'FU

AIR-3A 2'FU experimental SAS data
AIR-3A 2'FU Kratky plot
Sample: AIR-3A 2'FU dimer, 13 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 1.9 nm
Dmax 6.0 nm
VolumePorod 12 nm3

SASDAX7 – Aptamer AIR-3A G18U

AIR-3A G18U experimental SAS data
AIR-3A G18U Kratky plot
Sample: AIR-3A G18U tetramer, 25 kDa RNA
Buffer: water, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 6
Structure and target interaction of a G-quadruplex RNA-aptamer. RNA Biol 13(10):973-987 (2016)
Szameit K, Berg K, Kruspe S, Valentini E, Magbanua E, Kwiatkowski M, Chauvot de Beauchêne I, Krichel B, Schamoni K, Uetrecht C, Svergun DI, Schlüter H, Zacharias M, Hahn U
RgGuinier 2.5 nm
Dmax 8.3 nm
VolumePorod 24 nm3

SASDAC6 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data
PsrP functional binding region Rg histogram
Sample: Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer: PBS 5 % Glycerol, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2013 Feb 27
The BR domain of PsrP interacts with extracellular DNA to promote bacterial aggregation; structural insights into pneumococcal biofilm formation. Sci Rep 6:32371 (2016)
Schulte T, Mikaelsson C, Beaussart A, Kikhney A, Deshmukh M, Wolniak S, Pathak A, Ebel C, Löfling J, Fogolari F, Henriques-Normark B, Dufrêne YF, Svergun D, Nygren PÅ, Achour A
RgGuinier 2.0 nm
Dmax 7.8 nm
VolumePorod 38 nm3