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SASDC82 – Rab family protein CtRoco nucleotide free

Rab family protein experimental SAS data
DAMMIN model
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.0 nm
Dmax 18.4 nm
VolumePorod 440 nm3

SASDC92 – Rab family protein CtRoco GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 6.2 nm
Dmax 25.3 nm
VolumePorod 354 nm3

SASDCA2 – Rab family protein CtRoco GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.9 nm
Dmax 22.4 nm
VolumePorod 491 nm3

SASDCB2 – Rab family protein CtRoc-COR nucleotide free

Rab family protein experimental SAS data
DAMMIN model
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.4 nm
Dmax 10.0 nm
VolumePorod 211 nm3

SASDCC2 – Rab family protein CtRoc-COR GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µ M GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.5 nm
Dmax 10.0 nm
VolumePorod 197 nm3

SASDCD2 – Rab family protein CtRoc-COR GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 130 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Feb 1
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 3.4 nm
Dmax 10.5 nm
VolumePorod 154 nm3

SASDCE2 – Rab family protein CtRoco L487A GDP

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GDP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 17
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.9 nm
Dmax 21.8 nm
VolumePorod 469 nm3

SASDCF2 – Rab family protein CtRoco L487A GppNHp

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 6.0 nm
Dmax 23.0 nm
VolumePorod 436 nm3

SASDCG2 – Rab family protein CtRoco L487A

Rab family protein experimental SAS data
Rab family protein Kratky plot
Sample: Rab family protein dimer, 254 kDa Chlorobaculum tepidum protein
Buffer: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Feb 16
A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover. Nat Commun 8(1):1008 (2017)
Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W
RgGuinier 5.0 nm
Dmax 18.4 nm
VolumePorod 447 nm3

SASDD53 – Tetratrico peptide repeat domain of Bacterial cellulose synthesis subunit C

Bacterial cellulose synthesis subunit C experimental SAS data
DAMMIF model
Sample: Bacterial cellulose synthesis subunit C monomer, 71 kDa Enterobacter sp. CJF-002 protein
Buffer: 50 mM HEPES, 100 mM KCl, pH: 8
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Apr 24
Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C. Sci Rep 7(1):13018 (2017)
Nojima S, Fujishima A, Kato K, Ohuchi K, Shimizu N, Yonezawa K, Tajima K, Yao M
RgGuinier 5.1 nm
Dmax 18.5 nm
VolumePorod 115 nm3