Browse by ORGANISM: other species

SASDAB2 – Cytochrome c from equine heart

Cytochrome cHeme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c monomer, 12 kDa Equus caballus protein
Heme C monomer, 1 kDa
Buffer: 25 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Apr 8
Standard proteins
Cy M Jeffries
RgGuinier 1.3 nm
Dmax 3.7 nm
VolumePorod 12 nm3

SASDAC2 – Lysozyme in sodium acetate

Lysozyme C experimental SAS data
CRYSOL model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate, pH: 3.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 21
Standard proteins
Darja Ruskule
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 17 nm3

SASDAG2 – Lysozyme in sodium acetate

Lysozyme C experimental SAS data
GASBOR model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 40 mM Sodium Acetate, pH: 3.8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 21
Standard proteins
Darja Ruskule
RgGuinier 1.5 nm
Dmax 4.0 nm
VolumePorod 21 nm3

SASDAH2 – Myoglobin in PBS

Myoglobin experimental SAS data
CRYSOL model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 1.7 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAK2 – Myoglobin in PBS

Myoglobin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Myoglobin monomer, 17 kDa Equus caballus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 20
Standard proteins
Darja Ruskule
RgGuinier 1.6 nm
Dmax 5.0 nm
VolumePorod 32 nm3

SASDAL2 – Ovalbumin in PBS

Ovalbumin experimental SAS data
DAMMIN model
Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 18
Standard proteins
Darja Ruskule
RgGuinier 2.5 nm
Dmax 7.8 nm
VolumePorod 74 nm3

SASDAK6 – Glucose Isomerase

Xylose isomerase experimental SAS data
DAMMIF model
Sample: Xylose isomerase tetramer, 172 kDa Streptomyces rubiginosus protein
Buffer: PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Apr 24
Standard proteins
Erica Valentini
RgGuinier 3.4 nm
Dmax 9.7 nm
VolumePorod 293 nm3

SASDAB8 – Protein Interacting with C-kinase 1 (PICK1) LKV, dimer contribution (data decomposition).

PRKCA-binding protein experimental SAS data
Protein Interacting with C-kinase 1 (PICK1) LKV, dimer contribution (data decomposition). Rg histogram
Sample: PRKCA-binding protein dimer, 93 kDa Rattus norvegicus protein
Buffer: 50 mM Tris 125 mM NaCl 0.01 vol% reduced TX-100, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2015 May 11
Structure of Dimeric and Tetrameric Complexes of the BAR Domain Protein PICK1 Determined by Small-Angle X-Ray Scattering. Structure 23(7):1258-1270 (2015)
Karlsen ML, Thorsen TS, Johner N, Ammendrup-Johnsen I, Erlendsson S, Tian X, Simonsen JB, Høiberg-Nielsen R, Christensen NM, Khelashvili G, Streicher W, Teilum K, Vestergaard B, Weinstein H, Gether U, Arleth L, Madsen KL
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 205 nm3

SASDA58 – UL26N of pseudorabies virus

VP24 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: VP24 dimer, 53 kDa Suid herpesvirus 1 protein
Buffer: 50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23
Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease. PLoS Pathog 11(7):e1005045 (2015)
Zühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W
RgGuinier 2.6 nm
Dmax 10.6 nm
VolumePorod 73 nm3

SASDMX8 – Iron oxide nanoparticles (NP-N2) (30% of 9 kDa PEG tails)

Iron oxide nanoparticles (NP-N2) (30% of 9 kDa PEG tails) experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Iron oxide nanoparticles (NP-N2) (30% of 9 kDa PEG tails) 0, 5000 kDa
Buffer: 0.05 M Tris-HCl, 0.05 M NaCl, 0.01 M KCl, 0.005 M MgCl2, pH: 4.6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jun 23
Coat Protein-Dependent Behavior of Poly(ethylene glycol) Tails in Iron Oxide Core Virus-like Nanoparticles. ACS Appl Mater Interfaces 7(22):12089-98 (2015)
Malyutin AG, Cheng H, Sanchez-Felix OR, Carlson K, Stein BD, Konarev PV, Svergun DI, Dragnea B, Bronstein LM
Dmax 25.0 nm