Browse by ORGANISM: other species

SASDBM4 – The 1:1:9:1 crRNA:Cas6f:Cas7fv:Cas5fv CRISPR/Cas Type I-F long Cascade complex

Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family)Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822)Nonameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821)long-crRNA: CRISPR/Cas Type I-F Cascade component experimental SAS data
DAMMIF model
Sample: Cas6f: CRISPR/Cas Type I-F Cascade component (CRISPR-associated protein, Csy4 family) monomer, 21 kDa Shewanella putrefaciens protein
Cas5fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1822) monomer, 38 kDa Shewanella putrefaciens protein
Nonameric Cas7fv: CRISPR/Cas Type I-F Cascade component (Uncharacterized protein, Sputcn32_1821) nonamer, 335 kDa Shewanella putrefaciens protein
Long-crRNA: CRISPR/Cas Type I-F Cascade component monomer, 25 kDa RNA
Buffer: 50 mM HEPES 150 mM NaCl 1mM DTT 1mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jan 30
Modulating the Cascade architecture of a minimal Type I-F CRISPR-Cas system. Nucleic Acids Res 44(12):5872-82 (2016)
Gleditzsch D, Müller-Esparza H, Pausch P, Sharma K, Dwarakanath S, Urlaub H, Bange G, Randau L
RgGuinier 6.5 nm
Dmax 21.6 nm

SASDBV4 – Vaccinia virus MVA F1L antiapoptotic Bcl-2 viral protein

MVA F1L antiapoptotic Bcl-2 viral protein experimental SAS data
BUNCH model
Sample: MVA F1L antiapoptotic Bcl-2 viral protein dimer, 51 kDa Vaccinia virus protein
Buffer: 25 mM HEPES 150 mM NaCl 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 15
The N Terminus of the Vaccinia Virus Protein F1L Is an Intrinsically Unstructured Region That Is Not Involved in Apoptosis Regulation. J Biol Chem 291(28):14600-8 (2016)
Caria S, Marshall B, Burton RL, Campbell S, Pantaki-Eimany D, Hawkins CJ, Barry M, Kvansakul M
RgGuinier 3.4 nm
Dmax 16.2 nm
VolumePorod 74 nm3

SASDB54 – Leishmania braziliensis stress-induced protein sti1 (LbHop), full length construct

Stress-induced protein sti1 experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 monomer, 62 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS1 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 94 nm3

SASDB64 – Leishmania braziliensis stress-induced protein sti1 (LbHop TPR2A-TPR2B-DP2 construct)

Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) experimental SAS data
DAMFILT model
Sample: Stress-induced protein sti1 (Hop TPR2A-TPR2B-DP2 construct) monomer, 44 kDa Leishmania braziliensis protein
Buffer: 25 mM Tris 100 mM NaCl 1 mM EDTA 1 mM β-mercaptoethanol, pH: 7.5
Experiment: SAXS data collected at SAXS2 Beamline, Brazilian Synchrotron Light Laboratory on 2016 Feb 21
Low sequence identity but high structural and functional conservation: The case of Hsp70/Hsp90 organizing protein (Hop/Sti1) of Leishmania braziliensis. Arch Biochem Biophys 600:12-22 (2016)
Batista FAH, Seraphim TV, Santos CA, Gonzaga MR, Barbosa LRS, Ramos CHI, Borges JC
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 65 nm3

SASDAU8 – Immunosuppressive virulence protein YopM

Yersinia outer protein M (34-481) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 3.9 nm
Dmax 11.5 nm
VolumePorod 154 nm3

SASDAV8 – DEAD box helicase DDX3

DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 13
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 2.8 nm
Dmax 12.0 nm
VolumePorod 80 nm3

SASDAW8 – 2:1 YopM:DDX3 complex

Yersinia outer protein M (34-481)DEAD box RNA helicase DDX3 (51-418) experimental SAS data
SASREF model
Sample: Yersinia outer protein M (34-481) dimer, 101 kDa Yersinia enterocolitica protein
DEAD box RNA helicase DDX3 (51-418) monomer, 42 kDa Yersinia enterocolitica protein
Buffer: 50 mM HEPES 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 14
Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells. PLoS Pathog 12(6):e1005660 (2016)
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M
RgGuinier 4.0 nm
Dmax 15.0 nm
VolumePorod 190 nm3

SASDBU4 – Vaccinia primase D5 protein fragment containing the D5N and helicase domain

Primase D5 protein fragment containing the D5N and helicase domain experimental SAS data
DAMMIF model
Sample: Primase D5 protein fragment containing the D5N and helicase domain hexamer, 321 kDa Vaccinia virus protein
Buffer: 20 mM Tris 150 mM NaCL 5% glycerol 1mM DTT, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2014 Sep 26
Domain Organization of Vaccinia Virus Helicase-Primase D5. J Virol 90(9):4604-4613 (2016)
Hutin S, Ling WL, Round A, Effantin G, Reich S, Iseni F, Tarbouriech N, Schoehn G, Burmeister WP
RgGuinier 4.8 nm
Dmax 14.5 nm
VolumePorod 570 nm3

SASDB74 – CyaA Block I-V

Adenylate cyclase toxin Block I-V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block I-V monomer, 70 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P
RgGuinier 5.6 nm
Dmax 17.2 nm
VolumePorod 275 nm3

SASDB84 – CyaA Block V

Adenylate cyclase toxin Block V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block V monomer, 16 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 24 nm3