Browse by ORGANISM: other species

SASDAX8 – Ribokinase ThiM

Ribokinase ThiM experimental SAS data
CORAL model
Sample: Ribokinase ThiM trimer, 89 kDa Staphylococcus aureus protein
Buffer: 50 mM Potassium phosphate 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2010 Nov 19
Structure of ThiM from Vitamin B1 biosynthetic pathway of Staphylococcus aureus - Insights into a novel pro-drug approach addressing MRSA infections. Sci Rep 6:22871 (2016)
Drebes J, Künz M, Windshügel B, Kikhney AG, Müller IB, Eberle RJ, Oberthür D, Cang H, Svergun DI, Perbandt M, Betzel C, Wrenger C
RgGuinier 3.0 nm
Dmax 9.0 nm
VolumePorod 145 nm3

SASDAD7 – Structure of a complex between full length and truncated CTP1L endolysin

Endolysin  experimental SAS data
CRYSOL model
Sample: Endolysin , 33 kDa Clostridium phage phiCTP1 protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 17
Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem 291(10):4882-93 (2016)
Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R
RgGuinier 3.7 nm
Dmax 13.8 nm
VolumePorod 95 nm3

SASDAE7 – Structure of a complex between full length and truncated CS74L endolysin

Endolysin CS74L  experimental SAS data
DAMMIF model
Sample: Endolysin CS74L , 31 kDa Clostridium phage phi8074-B1 protein
Buffer: 20 mM HEPES, pH: 7.4
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Mar 17
Crystal Structure of the CTP1L Endolysin Reveals How Its Activity Is Regulated by a Secondary Translation Product. J Biol Chem 291(10):4882-93 (2016)
Dunne M, Leicht S, Krichel B, Mertens HD, Thompson A, Krijgsveld J, Svergun DI, Gómez-Torres N, Garde S, Uetrecht C, Narbad A, Mayer MJ, Meijers R
RgGuinier 3.6 nm
Dmax 14.0 nm
VolumePorod 79 nm3

SASDBS2 – Recombinant Tn antigen-binding lectin from Vatairea macrocarpa

Recombinant Tn antigen-binding lectin experimental SAS data
CORAL model
Sample: Recombinant Tn antigen-binding lectin tetramer, 105 kDa Vatairea macrocarpa protein
Buffer: 100 mM sodium phosphate 150 mM NaCl 5% (v/v) glycerol, pH: 5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 11
Structural characterization of a Vatairea macrocarpa lectin in complex with a tumor-associated antigen: A new tool for cancer research. Int J Biochem Cell Biol 72:27-39 (2016)
Sousa BL, Silva-Filho JC, Kumar P, Graewert MA, Pereira RI, Cunha RMS, Nascimento KS, Bezerra GA, Delatorre P, Djinovic-Carugo K, Nagano CS, Gruber K, Cavada BS
RgGuinier 3.2 nm
Dmax 9.5 nm
VolumePorod 168 nm3

SASDBC3 – RepB, the replication initiator protein of a promiscuous Streptococcal plasmid pMV158

Replication initiator protein of a promiscuous streptococcal plasmid pMV158. experimental SAS data
NONE model
Sample: Replication initiator protein of a promiscuous streptococcal plasmid pMV158. hexamer, Streptococcus sp. protein
Buffer: 10 mM TRIS 5 mM EDTA 1.0 M KCl, pH: 8.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jun 16
Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158. Sci Rep 6:20915 (2016)
Boer DR, Ruiz-Masó JA, Rueda M, Petoukhov MV, Machón C, Svergun DI, Orozco M, del Solar G, Coll M
RgGuinier 3.8 nm
Dmax 10.6 nm
VolumePorod 282 nm3

SASDBV2 – Zebrafish Arpin

Zebrafish (Danio rerio) Arpin experimental SAS data
Zebrafish (Danio rerio) Arpin Kratky plot
Sample: Zebrafish (Danio rerio) Arpin monomer, 25 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.7 nm
Dmax 13.8 nm
VolumePorod 47 nm3

SASDBW2 – Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16).

Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). experimental SAS data
Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). Kratky plot
Sample: Zebrafish (Danio rerio) Arpin truncated C-terminal mutant (delta-C 16). monomer, 24 kDa Danio rerio protein
Buffer: 50 mM HEPES 100mM NaCl 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2014 Dec 4
Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope. Structure 24(2):252-60 (2016)
Fetics S, Thureau A, Campanacci V, Aumont-Nicaise M, Dang I, Gautreau A, Pérez J, Cherfils J
RgGuinier 2.2 nm
Dmax 11.3 nm
VolumePorod 37 nm3

SASDBQ3 – Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164)

Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) experimental SAS data
DAMMIF model
Sample: Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) monomer, 18 kDa Middle East respiratory … protein
Buffer: 10 mM HEPES 300 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Oct 5
Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering. Acta Crystallogr D Struct Biol 72(Pt 2):192-202 (2016)
Papageorgiou N, Lichière J, Baklouti A, Ferron F, Sévajol M, Canard B, Coutard B
RgGuinier 2.0 nm
Dmax 8.0 nm
VolumePorod 28 nm3

SASDL67 – Mutation of the Active Site Residues of Thermus thermophilus 3‑Isopropylmalate Dehydrogenase

3-isopropylmalate dehydrogenase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 3-isopropylmalate dehydrogenase dimer, 73 kDa Thermus thermophilus protein
Buffer: 25 mM MOPS/NaOH, pH: 7.6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2012 Nov 16
Dual Role of the Active Site Residues of Thermus thermophilus 3-Isopropylmalate Dehydrogenase: Chemical Catalysis and Domain Closure. Biochemistry 55(3):560-74 (2016)
Gráczer É, Szimler T, Garamszegi A, Konarev PV, Lábas A, Oláh J, Palló A, Svergun DI, Merli A, Závodszky P, Weiss MS, Vas M
RgGuinier 2.8 nm

SASDB44 – Aureochrome 1a from P. tricornutum, amino acids 148-378 (N-terminal truncation), dark state

Aureochrome 1a (N-terminally truncated) experimental SAS data
DAMMIN model
Sample: Aureochrome 1a (N-terminally truncated) dimer, 53 kDa Phaeodactylum tricornutum protein
Buffer: 20 mM HEPES 100 mM NaCl 10 mM MgCl2 5% w/v glycerol, pH: 7.5
Experiment: SAXS data collected at cSAXS, Swiss Light Source on 2015 Mar 11
Blue light-induced LOV domain dimerization enhances the affinity of Aureochrome 1a for its target DNA sequence. Elife 5:e11860 (2016)
Heintz U, Schlichting I
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 90 nm3