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SASDS66 – Solution conformation of Harpin HrpZ2 protein from Pseudomonas syringae, a plant pathogen

Harpin Z2 experimental SAS data
CORAL model
Sample: Harpin Z2 monomer, 37 kDa Pseudomonas syringae strain … protein
Buffer: 10 mM MES, 100 mM NaF, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Centre for Cellular and Molecular Biology on 2023 May 1
Solution conformation of HrpZ2 protein from Pseudomonas syringae
Arpita Goswami
RgGuinier 3.1 nm
Dmax 8.8 nm
VolumePorod 64 nm3

SASDS37 – Oligomerization initiating solution conformation of Harpin HrpZ2 protein from Pseudomonas syringae, a plant pathogen

Harpin Z2 experimental SAS data
CORAL model
Sample: Harpin Z2 monomer, 37 kDa Pseudomonas syringae strain … protein
Buffer: 10 mM MES, 100 mM NaF, pH: 6.2
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Centre for Cellular and Molecular Biology on 2023 May 1
Solution conformation of HrpZ2 protein from Pseudomonas syringae
Arpita Goswami
RgGuinier 3.2 nm
Dmax 11.4 nm
VolumePorod 70 nm3

SASDCN8 – SAXS data of Legionella pneumophila phosphocholine hydrolase Lem3(19-570)

Phosphocholine hydrolase Lem3 experimental SAS data
Phosphocholine hydrolase Lem3 Kratky plot
Sample: Phosphocholine hydrolase Lem3 monomer, 63 kDa Legionella pneumophila subsp. … protein
Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 20
The structural analysis of dephosphocholinase Legionella pneumophila Lem3
Wenhua Zhang
RgGuinier 3.5 nm
Dmax 12.2 nm
VolumePorod 95 nm3

SASDTW8 – Staphylococcus aureus Fatty Acid Kinase A

Uncharacterized protein SAUSA300_1119 experimental SAS data
DAMMIF model
Sample: Uncharacterized protein SAUSA300_1119 dimer, 125 kDa Staphylococcus aureus (strain … protein
Buffer: 50 mM Tris, 150 mM KCl, 1 mM TCEP, 5% glycerol, pH: 7.4
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 5
Molecular insights into the structure and function of the Staphylococcus aureus fatty acid kinase. J Biol Chem 300(12):107920 (2024)
Myers MJ, Xu Z, Ryan BJ, DeMars ZR, Ridder MJ, Johnson DK, Krute CN, Flynn TS, Kashipathy MM, Battaile KP, Schnicker N, Lovell S, Freudenthal BD, Bose JL
RgGuinier 4.3 nm
Dmax 16.0 nm
VolumePorod 219 nm3

SASDV75 – S9 carboxypeptidase from Bacillus subtilis (4 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
Chandravanshi K, Singh R, Kumar A, Bhange GN, Kumar A, Makde RD
RgGuinier 5.2 nm
Dmax 15.9 nm
VolumePorod 439 nm3

SASDV85 – S9 carboxypeptidase from Bacillus subtilis (6 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
Chandravanshi K, Singh R, Kumar A, Bhange GN, Kumar A, Makde RD
RgGuinier 5.2 nm
Dmax 14.7 nm
VolumePorod 435 nm3

SASDV95 – S9 carboxypeptidase from Bacillus subtilis (8 mg/ml)

Putative acylaminoacyl-peptidase experimental SAS data
GASBOR model
Sample: Putative acylaminoacyl-peptidase tetramer, 293 kDa Bacillus spizizenii (strain … protein
Buffer: 10 mM Tris-HCl, 135 mM NaCl, pH: 8
Experiment: SAXS data collected at BL-18, INDUS-2 on 2024 Mar 18
Structural adaptations for carboxypeptidase activity in putative S9 acylaminoacyl peptidase from Bacillus subtilis. Int J Biol Macromol :136734 (2024)
Chandravanshi K, Singh R, Kumar A, Bhange GN, Kumar A, Makde RD
RgGuinier 5.2 nm
Dmax 15.3 nm
VolumePorod 446 nm3

SASDU52 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, apo form

Cereblon-midi experimental SAS data
SWISSMODEL model
Sample: Cereblon-midi monomer, 37 kDa protein
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
Kroupova A, Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 61 nm3

SASDGE6 – G-quadruplex DNA from Hepatitis B virus (wild type)

G-quadrupex experimental SAS data
DAMFILT model
Sample: G-quadrupex monomer, 6 kDa Hepatitis B virus DNA
Buffer: 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 15
G-quadruplex from HBV genome
Trushar Patel
RgGuinier 1.7 nm
Dmax 4.0 nm
VolumePorod 13 nm3

SASDGF6 – G-quadruplex DNA from Hepatitis B virus (mutant)

G-quadruplex mutant experimental SAS data
DAMFILT model
Sample: G-quadruplex mutant monomer, 6 kDa Hepatitis B virus DNA
Buffer: 20 mM HEPES, 100 mM KCl, 1 mM EDTA, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jan 15
G-quadruplex from HBV genome
Trushar Patel
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 11 nm3